Substituted bicyclic derivatives useful as anticancer agents

专利摘要:
The present invention relates to a compound of formula (1), or a pharmaceutically acceptable salt or solvate thereof: Formula 1 Where A, X, R 1 , R 3 and R 4 are each as defined herein. The present invention also relates to a method of treating abnormal cell growth in a mammal by administering a compound of Formula 1 to a mammal, and to a pharmaceutical composition for the treatment of such a disease containing the compound of Formula 1. The present invention also relates to a process for preparing the compound of formula (1).
公开号:KR20010101774A
申请号:KR1020017009539
申请日:1999-12-06
公开日:2001-11-14
发明作者:케쓰존찰스；톰노마잭쾰린；리우젱규；콕스에릭데이비드；브하타차랴사미트쿠마르；모리스조엘
申请人:실버스타인 아써 에이.；화이자 프로덕츠 인코포레이티드；
IPC主号:

专利说明:

Substituted bicyclic derivatives useful as anticancer agents {SUBSTITUTED BICYCLIC DERIVATIVES USEFUL AS ANTICANCER AGENTS}
[2] Cells are known to be cancerous by the transformation of a portion of their DNA into an oncogene (ie, a gene that, when activated, forms malignant tumor cells). Many oncogenes encode proteins that are aberrant tyrosine kinases that can induce cell transformation. Alternatively, usually overexpression of proto-tumor tyrosine kinases also results in proliferative disease, sometimes with a malignant phenotype.
[3] Receptor tyrosine kinases exist throughout the cell membrane and act as kinases to extracellular binding domains for growth factors (e.g. epidermal growth factor), transmembrane domains, and phosphorylate specific tyrosine residues in proteins. It is an enzyme with an intracellular part that affects proliferation. Other receptor tyrosine kinases include c-erbB-2, c-met, tie-2, PDGFr, FGFr and VEGFR. Such kinases are often known to be ideally expressed in common human cancers such as breast cancer, gastrointestinal cancer (eg colon cancer, rectal cancer or gastric cancer), leukemia, and ovarian cancer, bronchial cancer or pancreatic cancer. In addition, epidermal growth factor receptors (EGFRs) with tyrosine kinase activity are also found in many human cancers, such as brain tumors, lung tumors, squamous cell tumors, bladder tumors, intestinal tumors, breast tumors. And mutated and / or overexpressed in head and neck tumors, esophageal tumors, gynecological tumors and thyroid tumors.
[4] Thus, inhibitors of receptor tyrosine kinases are known to be useful as selective inhibitors of growth of mammalian cancer cells. For example, erbstatin, a tyrosine kinase inhibitor, is an athymic of transplanted human breast cancer that expresses epidermal growth factor receptor tyrosine kinase (EGFR) but does not affect the growth of other carcinomas that do not express EGF receptors. It selectively inhibits growth in athymic nude mice. Thus, the compounds of the invention, which are selective inhibitors of specific receptor tyrosine kinases, are useful for treating abnormal cell growth, particularly cancer, in mammals. In addition to receptor tyrosine kinases, the compounds of the present invention may also exhibit inhibitory activity against many other non-receptor tyrosine kinases (eg lck, src, abl) or serine / threonine kinases (eg cyclin dependent kinases).
[5] Various other compounds, such as styrene derivatives, have also been shown to have tyrosine kinase inhibitory properties. More recently, five European patent applications: EP 0 566 226 A1 (published October 20, 1993), EP 0 602 851 A1 (published June 22, 1994), EP 0 635 507 A1, published January 25, 1995, EP 0 635 498 A1, published January 25, 1995, and EP 0 520 722 A1, published December 30, 1992. Mentions certain bicyclic derivatives, in particular quinazoline derivatives, having anticancer properties due to tyrosine kinase inhibition. International patent application WO 92/20642 (published November 26, 1992) also refers to certain bis-mono and bicyclic aryl and heteroaryl compounds as tyrosine kinase inhibitors useful for inhibiting abnormal cell proliferation. International Patent Application WO 96/16960 (published June 6, 1996), International Patent Application WO 96/09294 (published March 6, 1996), International Patent Application WO 97/30034 (published) Date: August 21, 1997), International Patent Application WO 98/02434 (published January 22, 1998), International Patent Application WO 98/02437 (published January 22, 1998); International patent application WO 98/02438 (published: January 22, 1998) also mentions bicyclic heteroaromatic derivatives substituted as tyrosine kinase inhibitors useful for this purpose.
[6] Summary of the Invention
[7] The present invention relates to a compound of formula (1), or a pharmaceutically acceptable salt or solvate thereof:
[8]
[9] Where
[10] X is N or CH;
[11] A optionally contains 1 to 4 heteroatoms which may be the same or different and are selected from -N (R ¹ )-, O, and S (O) _j , where j is an integer from 0 to 2 A condensed 5, 6 or 7 membered ring containing a total of 1 to 3 double bonds, including those in the condensed pyridine or pyrimidine ring,
[12] Wherein if the double bond comprises said selective nitrogen moiety -N (R ¹ )-then there is no R ¹ group bonded to nitrogen provided that the condensed ring does not form part of the purine and has two adjacent O or Does not contain an S (O) _j atom and the carbon atom of the A residue is optionally substituted with 1 to 3 R ⁵ groups;
[13] R ¹ and R ² are each independently H or C ₁ -C ₆ alkyl;
[14] R ³ is-(CR ¹ R ² ) _m -R ⁸ , wherein m is 0 or 1;
[15] R ¹ and R ³ are together Forms a group of which is optionally substituted with one to three R ⁵ groups;
[16] R ⁴ is-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _t R ⁹ ,-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _t R ⁹ ,- C = NOR ¹² or —X ¹ -R ¹² where m is an integer from 0 to 3, t is an integer from 0 to 5, and X ¹ is an azetidine, oxetane or C ₃ -C ₄ carbocyclic group Is a group derived from) or-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _k R ¹³ or-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _k R ¹³ (where k is an integer from 1 to 3, m is an integer from 0 to 3), or-(CR ¹ R ² ) _t R ⁹ (where t is an integer from 0 to 5, the attachment point to R ⁹ is through a carbon atom of the R ⁹ groups shown below);
[17] Each R ⁵ is independently halo, hydroxy, —NR ¹ R ² , C ₁ -C ₆ alkyl, trifluoromethyl, C ₁ -C ₆ alkoxy, trifluoromethoxy, -C (O) R ⁶ ,- CO ₂ R ⁶ , -NR ⁶ C (O) R ¹ , -C (O) NR ⁶ R ⁷ , -SO ₂ NR ⁶ R ⁷ , -NR ⁶ C (O) NR ⁷ R ¹ and -NR ⁶ C ( O) OR ⁷ ;
[18] R ⁶ and R ⁷ are each independently H, C ₁ -C ₆ alkyl,-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _t (4- to 10-membered heterocycle) Wherein t is an integer from 0 to 5 and one or two ring carbon atoms of the heterocyclic group are optionally substituted with an oxo (═O) moiety and alkyl of the above R ⁶ and R ⁷ groups , Aryl and heterocyclic moieties include halo, cyano, nitro, -NR ¹ R ² , trifluoromethyl, trifluoromethoxy, C ₁ -C ₆ alkyl, C ₂ -C ₆ alkenyl, C ₂ -C ₆ Optionally substituted with 1 to 3 substituents independently selected from alkynyl, hydroxy and C ₁ -C ₆ alkoxy;
[19] R ⁸ is independently-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _t (4 to 10 membered heterocycle), wherein t is an integer from 0 to 5, One or two ring carbon atoms of the heterocyclic group are optionally substituted with oxo (═O) residues), wherein the R ⁸ groups are each optionally substituted with 1 to 5 R ¹⁰ groups;
[20] R ⁹ is a non-aromatic monocyclic ring, a condensed or crosslinked bicyclic ring, or a spiro ring ring, wherein the ring has 0 to 3 carbon atoms N, O, S (O) _j , wherein j is 0 to 2 Integer) and 3 to 12 carbon atoms optionally substituted with a hetero residue independently selected from -NR ^12- , provided that only two O atoms, two S (O) _j residues, an O atom and S (O ) _j residues, N and S atoms, or N and O atoms are not directly bonded to each other in the ring, and the carbon atoms of the ring are optionally substituted with 1 or 2 R ¹¹ groups;
[21] Each R ¹⁰ is independently halo, cyano, nitro, trifluoromethoxy, trifluoromethyl, azido, hydroxy, C ₁ -C ₆ alkoxy, C ₁ -C ₁₀ alkyl, C ₂ -C ₆ alkenyl , C ₂ -C ₆ alkynyl, -C (O) R ⁶ , -C (O) OR ⁶ , -OC (O) R ⁶ , -NR ⁶ C (O) R ⁷ , -NR ⁶ C (O) NR ¹ R ⁷ , -NR ⁶ C (O) OR ⁷ , -C (O) NR ⁶ R ⁷ , -NR ⁶ R ⁷ , -NR ⁶ OR ⁷ , -SO ₂ NR ⁶ R ⁷ , -S (O) _j (C ₁ -C ₆ alkyl), where j is an integer from 0 to 2,-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl),-(CR ¹ R ² ) _t (4 to 10 membered heterocycle),-(CR ¹ R ² ) _q C (O) (CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl),-(CR ¹ R ² ) _q C (O) (CR ¹ R ² ) _t (4 to 10 membered heterocycle),-(CR ¹ R ² ) _t O (CR ¹ R ² ) _q (C ₆ -C ₁₀ aryl),-(CR ¹ R ² ) _t O (CR ¹ R ² ) _q (4 to 10 membered heterocycle),-(CR ¹ R ² ) _q S (O) _j (CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _q S (O) _j (CR ¹ R ² ) _t (4 to 10 membered heterocycle), wherein j is 0 to 2 and q and t are each independently an integer of 0 to 5, and
[22] One or two ring carbon atoms of the heterocyclic residue of the R ¹⁰ group is optionally substituted with an oxo (═O) residue,
[23] Alkyl, alkenyl, alkynyl, aryl and heterocyclic moieties of the above R ¹⁰ group include halo, cyano, nitro, trifluoromethyl, trifluoromethoxy, azido, -OR ⁶ , -C (O) R ⁶ , -C (O) OR ⁶ , -OC (O) R ⁶ , -NR ⁶ C (O) R ⁷ , -C (O) NR ⁶ R ⁷ , -NR ⁶ R ⁷ , -NR ⁶ OR ⁷ , C ₁ -C ₆ alkyl, C ₂ -C ₆ alkenyl, C ₂ -C ₆ alkynyl,-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _t (4 Optionally substituted with 1 to 3 substituents independently selected from: 10-membered heterocycle), wherein t is an integer from 0 to 5;
[24] R ¹¹ are each independently -R ¹² , -OR ¹ , -NR ¹ R ² , -NR ⁶ C (O) R ⁷ , -NR ⁶ C (O) NR ⁷ R ¹ , -NR ⁶ C (O) OR ⁷ and —NR ⁶ SO ₂ NR ⁷ R ¹ , or substitute two hydrogen atoms on carbon to form an oxo (C═O) group;
[25] R ¹² is R ⁶ , —C (O) R ⁶ or —SO ₂ R ⁶ , —C (O) NR ⁶ R ⁷ , —SO ₂ NR ⁶ R ⁷ or —CO ₂ R ⁶ ;
[26] R ¹³ is —NR ¹ R ¹² or —OR ¹² ;
[27] Wherein any substituent described above including a halogeno, SO or SO ₂ group, or a CH ₃ (methyl), CH ₂ (methylene) or CH (methine) group not bonded to an N, O or S atom Optionally has a substituent selected from hydroxy, halo, C ₁ -C ₄ alkyl, C ₁ -C ₄ alkoxy and —NR ¹ R ² in the group.
[28] In certain embodiments of the invention, the A moiety of the compound of Formula 1 is selected from the following formulas having R ⁴ groups as substituents and optionally having 1 to 3 R ⁵ groups as substituents:
[29]
[30] In another specific embodiment of the invention, the A moiety of the compound of formula 1 is selected from the following formulas having R ⁴ groups as substituents and optionally 1 to 3 R ⁵ groups as substituents:
[31]
[32] In another specific embodiment of the invention, the A moiety of the compound of formula 1 is selected from the following formulas having R ⁴ groups as substituents and optionally 1 to 3 R ⁵ groups as substituents:
[33]
[34] In another specific embodiment of the invention, the A moiety of the compound of formula 1 is selected from the following formulas having R ⁴ groups as substituents and optionally 1 to 3 R ⁵ groups as substituents:
[35]
[36] Other specific embodiments of compounds of Formula 1 include compounds wherein A is of the formula: having R ⁴ groups as substituents and optionally having 1 to 3 R ⁵ groups as substituents:
[37]
[38] Another specific embodiment of the compounds of formula (1) R ⁴ is _{^{- (CR 1 R 2) m}} -C≡C- (CR 1 R 2) t R 9 ( where, m is an integer from 0 to 3, t is from 0 to It is an integer of 5).
[39] In certain other embodiments of compounds of Formula 1, R ⁴ is-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _t R ⁹ , wherein m is an integer from 0 to 3, t is from 0 to It is an integer of 5).
[40] Another specific embodiment of the compounds of formula (1) R ⁴ is _{^{- (CR 1 R 2) m}} -C≡C- (CR 1 R 2) k R 13 or _{^{- (CR 1 R 2) m}} -C = C- (CR ¹ R ² ) _k R ¹³ wherein m is an integer from 0 to 3 and k is an integer from 1 to 3.
[41] Another specific embodiment of compounds of Formula 1 is a compound wherein R ⁴ is -C = NOR ¹² or -X ¹ -R ¹² , wherein X ¹ is a divalent group derived from an azetidine, oxetane or C ₃ -C ₄ carbocyclic group a), or - it includes those wherein _{^{(CR 1 R 2) t R}} 9 ( where the attachment point to R ⁹ is through a carbon atom in R ^9).
[42] Other specific embodiments of compounds of Formula 1 include wherein R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl) ,-(CR ¹ R ² ) _t (indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), wherein t is from 0 to 5 Integers), wherein each of the R ⁸ groups is optionally substituted with 1 to 5 R ¹⁰ groups.
[43] Other particular embodiments of compounds of Formula 1 include compounds wherein R ⁹ is a 4-10 membered heterocyclic group having 1 to 3 hetero moieties as described in Formula 1 and optionally substituted with 1 or 2 R ¹¹ groups .
[44] Preferred compounds include compounds selected from the group consisting of:
[45] Acetic acid 3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -allyl ester;
[46] (1-benzenesulfonyl-1H-indol-5-yl)-[6- [3- (4-methyl-piperazin-1-yl) -prop-1-ynyl] -quinazolin-4-yl] Amines;
[47] (1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-pyrrolidin-1-yl-prop-1-ynyl] -quinazolin-4-yl] -amine;
[48] 4- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol;
[49] (1-benzenesulfonyl-1H-indol-5-yl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl] -amine;
[50] [6- (4-Amino-tetrahydro-pyran-4-ylethynyl) -quinazolin-4-yl]-(1-benzenesulfonyl-1H-indol-5-yl) -amine;
[51] 1-methyl-4- [4- [3-methyl-4- (pyridin-2-ylmethoxy) -phenylamino] -quinazolin-6-ylethynyl] -piperidin-4-ol;
[52] 1- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol;
[53] 4- [4- [4- (1-phenyl-ethoxy) -phenylamino] -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol;
[54] 1- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -4,4-dimethyl-pent-1-yn-3-ol;
[55] 4,4-dimethyl-1- [4- [4- (1-phenyl-ethoxy) -phenylamino] -quinazolin-6-yl] -pent-1-yn-3-ol;
[56] 3- [4- [1- (Propan-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol;
[57] 1-methyl-3- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[58] 3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[59] 3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-yn-1-ol;
[60] 5- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one;
[61] 4-amino-1- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol;
[62] 4-amino-1- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol;
[63] 3- [2- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol; And
[64] Pharmaceutically acceptable salts and solvates of these compounds.
[65] According to the invention, most preferred compounds include compounds selected from the group consisting of:
[66] (+)-(3-Methyl-4-phenoxy-phenyl)-(6-piperidin-3 (R) -ylethynyl-quinazolin-4-yl) -amine;
[67] (-)-(3-methyl-4-phenoxy-phenyl)-(6-piperidin-3 (S) -ylethynyl-quinazolin-4-yl) -amine;
[68] 3- (S)-[4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidine-1-carboxylic acid methylamide;
[69] 3- (S)-[4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidine-1-carboxylic acid methylamide;
[70] (3-Methyl-4-phenoxy-phenyl)-(6-pyrrolidin-3-ylethynyl-quinazolin-4-yl) -amine;
[71] 3- [4- (5-Methyl-6-phenoxy-pyridin-3-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[72] (-)-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[73] (+)-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[74] 4- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol;
[75] [6- [1- (2-methoxy-ethyl) -piperidin-3-ylethynyl] -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine;
[76] [4- (2-Fluoro-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine;
[77] [4- (3-Fluoro-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine;
[78] (6-azetidin-3-ylethynyl-quinazolin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine;
[79] 3- [4- [4- (2-fluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol;
[80] 3- [4- [4- (3-fluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol;
[81] [4- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol;
[82] (3-Chloro-4-phenoxy-phenyl)-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine;
[83] 3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol;
[84] (3-Chloro-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl) -amine;
[85] 3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol;
[86] 3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-7-ylethynyl] -piperidin-3-ol; And
[87] Pharmaceutically acceptable salts and solvates of these compounds.
[88] Other preferred compounds include compounds selected from the group consisting of:
[89] N- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
[90] N- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
[91] (3- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -3-aza-bicyclo [3.1.0] Hex-6-yl) -methanol;
[92] 4- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperazin-1-carboxylic acid methylamide;
[93] [6- [3- (1,1-Dioxo-1-thiomorpholin-4-yl) -prop-1-ynyl] -quinazolin-4-yl]-(3-methyl-4-phenoxy -Phenyl) -amine;
[94] 1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperidin-4-ol;
[95] N- [1-Methyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
[96] N-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-methyl-prop-2-ynyl] -acetamide;
[97] N- [1,1-Dimethyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
[98] 4- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol;
[99] 3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[100] 3- [4- (3-bromo-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[101] 3- [4- (4-benzenesulfonyl-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[102] 3- [4- (4-cyclohexyloxy-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[103] 2-methyl-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-2-ol;
[104] 2-amino-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-1-ol;
[105] 3- [4- (3-Methyl-4-phenylsulfanyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol; And
[106] Pharmaceutically acceptable salts and solvates of these compounds.
[107] Other preferred compounds include compounds selected from the group consisting of:
[108] 3- [4- (3-chloro-4-fluoro-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[109] 3- [4- (3-ethynyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[110] (3-methyl-4-phenoxy-phenyl)-[6- (1-methyl-piperidin-3-ylethynyl) -quinazolin-4-yl] -amine;
[111] (3-methyl-4-phenoxy-phenyl)-[6- (2-piperidin-3-yl-ethyl) -quinazolin-4-yl] -amine;
[112] 3- [2- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol;
[113] 3- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
[114] 3-oxo-5- (4-pyrrolidin-1-yl-butyl) -1,2,3,5-tetrahydro-benzo [4,5] imidazo [1,2-a] pyridine-4- Carboxylic acid benzylamide; And
[115] Pharmaceutically acceptable salts and solvates of these compounds.
[116] In addition, the present invention comprises administering to a mammal, including a human, a compound of formula 1, or a pharmaceutically acceptable salt or solvate thereof, in an amount effective to treat abnormal cell growth. It relates to a method for treating abnormal cell growth. In one embodiment of this method, abnormal cell growth is cancer, such as lung cancer, bone cancer, pancreatic cancer, skin cancer, head or neck cancer, skin or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer, cancer of the anal region, gastric cancer, colon cancer, breast cancer, fallopian tube carcinoma, endometrial carcinoma, cervical carcinoma, vaginal carcinoma, vulvar carcinoma, Hodgkin's disease, esophageal cancer, small intestine cancer, endocrine cancer, thyroid cancer, parathyroid cancer, adrenal gland cancer, Soft tissue sarcoma, urethral cancer, penile cancer, prostate cancer, chronic or acute leukemia, lymphocyte lymphoma, bladder cancer, kidney or ureter cancer, renal cell carcinoma, renal carcinoma, central nervous system neoplasm, primary CNS lymphoma , Spinal cord tumor, brain stem glioma, pituitary adenoma, or one or more combinations of these cancers. In another embodiment of the method, the abnormal cell growth is a benign proliferative disease, including but not limited to psoriasis, benign prostatic hyperplasia or restenosis.
[117] In addition, the present invention provides an effective amount of a compound of formula 1, or a pharmaceutically acceptable salt or solvate thereof, to treat abnormal cell growth, including mitosis inhibitors, alkylating agents, anti-metabolites, intercalating antibiotics, growth factor inhibitors. To a mammal with an anti-tumor agent selected from the group consisting of cell cycle inhibitors, enzymes, tooisomerase inhibitors, biological response modifiers, antibodies, cytotoxic agents, anti-hormones and anti-androgens. It comprises a method for treating abnormal cell growth of said mammal.
[118] The present invention also includes a human, comprising an amount of a compound of formula 1 as described above, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier, in an amount effective to treat abnormal cell growth. A pharmaceutical composition for treating abnormal cell growth in a mammal. In one embodiment of this composition, the abnormal cell growth is cancer, such as lung cancer, bone cancer, pancreatic cancer, skin cancer, head or neck cancer, skin or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer, anal muscle cancer, gastric cancer, colon cancer, Breast cancer, fallopian tube carcinoma, endometrial carcinoma, cervical carcinoma, vaginal carcinoma, vulvar carcinoma, Hodgkin's disease, esophageal cancer, small intestine cancer, endocrine cancer, thyroid cancer, parathyroid cancer, adrenal gland cancer, soft tissue sarcoma, urethral cancer, penis cancer, prostate cancer , Chronic or acute leukemia, lymphocyte lymphoma, bladder cancer, kidney or ureter cancer, renal cell carcinoma, pyelone carcinoma, CNS neoplasia, primary CNS lymphoma, spinal cord tumor, brain stem glioma, pituitary adenoma, or a combination of one or more of these cancers Including but not limited to. In another embodiment of the pharmaceutical composition, the abnormal cell growth is a benign proliferative disease, including but not limited to psoriasis, benign prostatic hyperplasia or restenosis.
[119] In addition, the present invention provides a pharmaceutically acceptable carrier and mitosis inhibitor, alkylating agent, anti- pharmaceutically acceptable compound of Formula 1, or a pharmaceutically acceptable salt or solvate thereof, in an amount effective to treat abnormal cell growth. Human with an anti-tumor agent selected from the group consisting of metabolites, intercalating antibiotics, growth factor inhibitors, cell cycle inhibitors, enzymes, topoisomerase inhibitors, biological response modifiers, anti-hormonal agents and anti-androgens It relates to a pharmaceutical composition for treating abnormal cell growth in a mammal, including.
[120] In addition, the present invention, (a) the compound of formula 11 or formula 2 is reacted with the compound of formula 3, or (b) the compound of formula 7 is reacted with the compound of formula 3 It relates to a method of preparing a compound of formula 1 and its pharmaceutically acceptable salts or solvates comprising obtaining:
[121] Formula 1
[122]
[123] Where
[124] A, X, R ¹ , R ⁴ and R ³ are as defined above.
[125]
[126] Where
[127] Z is a leaving group and A, X, R ¹ , R ⁴ and R ³ are as defined above.
[128]
[129] Where
[130] X, R ¹ , A and R ³ are as defined above and Z ¹ is an activation group.
[131]
[132] Where
[133] Z ¹ , X, R ¹ , A and R ³ are as defined above and Z ¹ is converted to an R ⁴ group.
[134] As used herein, the term “abnormal cell growth” refers to cell growth (eg, loss of contact inhibition) that is independent of normal regulatory mechanisms unless otherwise indicated. These include (1) tumor cells (tumors) that proliferate by expressing mutated tyrosine kinases or by overexpressing receptor tyrosine kinases; (2) benign and malignant cells of other proliferative diseases in which aberrant tyrosine kinase activation occurs; (4) any tumor that proliferates by receptor tyrosine kinase; (5) any tumor that proliferates by aberrant serine / threonine kinase activation; And (6) benign growth of other proliferative diseases in which aberrant serine / threonine kinase activation occurs and abnormal growth of malignant cells.
[135] As used herein, the term “treating” means, unless otherwise indicated, to reverse, alleviate, inhibit or prevent the disease or condition to which this term applies or one or more symptoms of such disease or condition. The term "treatment" as used herein, unless otherwise indicated, refers to the therapeutic behavior of "treating" as defined immediately above.
[136] As used herein, the term “halo” means fluoro, chloro, bromo or iodo unless otherwise indicated. Preferred halo groups are fluoro, chloro and bromo.
[137] As used herein, unless otherwise indicated, the term "alkyl" refers to a saturated monovalent hydrocarbon radical having linear or branched or cyclic moieties (including condensed and crosslinked bicyclic and helical moieties), or combinations of these moieties. It includes. In order for the alkyl group to have a cyclic moiety, the group must have at least 3 carbon atoms.
[138] The term "alkenyl" as used herein, unless otherwise indicated, is an alkyl moiety having at least one carbon-carbon double bond and comprising the E and Z isomers of the alkenyl moiety, wherein alkyl is as defined above. ).
[139] The term "alkynyl" as used herein, unless otherwise indicated, includes alkyl moieties having one or more carbon-carbon triple bonds, wherein alkyl is as defined above.
[140] The term "alkoxy" as used herein, unless otherwise indicated, includes O-alkyl groups, wherein alkyl is as defined above.
[141] The term "aryl" as used herein, unless otherwise indicated, includes organic radicals derived by removing one hydrogen from an aromatic hydrocarbon, such as phenyl or naphthyl.
[142] As used herein, the term “4- to 10-membered heterocycle”, unless otherwise indicated, includes aromatic and non-aromatic heterocyclic groups containing 1 to 4 heteroatoms each selected from the group consisting of O, S and N, wherein Heterocyclic groups each have 4 to 10 atoms in the ring system, provided that the rings of the groups do not contain two adjacent O or S atoms. Non-aromatic heterocyclic groups include groups having only 4 atoms in the ring system, while aromatic heterocyclic groups must have 5 or more atoms in the ring system. Heterocyclic groups include benzo-condensed ring systems. An example of a four-membered heterocyclic group is azetidinyl (derived from azetidine). An example of a 5 membered heterocyclic group is thiazolyl and an example of a 10 membered heterocyclic group is quinolinyl. Examples of non-aromatic heterocyclic groups include pyrrolidinyl, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, dihydropyranyl, tetrahydrothiopyranyl, piperidino, morpholino , Thiomorpholino, thioxanyl, piperazinyl, azetidinyl, oxetanyl, thietanyl, homopiperidinyl, oxepanyl, thiepanyl, oxazinyl, diazepinyl, thiazinyl, 1 , 2,3,6-tetrahydropyridinyl, 2-pyrrolinyl, 3-pyrrolinyl, indolinyl, 2H-pyranyl, 4H-pyranyl, dioxanyl, 1,3-dioxolanyl, pyra Zolinyl, dithianyl, dithiolanyl, dihydropyranyl, dihydrothienyl, dihydrofuranyl, pyrazolidinyl, imidazolinyl, imidazolidinyl, 3-azabicyclo [3.1.0] hexanyl , 3-azabicyclo [4.1.0] heptanyl, azabicyclo [2.2.2] hexanyl, 3H-indolyl and quinolinzinyl. Examples of aromatic heterocyclic groups include pyridinyl, imidazolyl, pyrimidinyl, pyrazolyl, triazolyl, pyrazinyl, tetrazolyl, furyl, thienyl, isooxazolyl, thiazolyl, oxazolyl, isothiazolyl, pi Rollyl, quinolinyl, isoquinolinyl, indolyl, benzimidazolyl, benzofuranyl, cinnaolinyl, indazolyl, indolinyl, phthalazinyl, pyridazinyl, triazinyl, isoindolyl, fr Teridinyl, furinyl, oxadizolyl, thiadiazolyl, furazanyl, benzofurazanyl, benzothiophenyl, benzothiazolyl, benzoxazolyl, quinazolinyl, quinoxalinyl, naphthyridinyl and furopyridinyl . The groups as derived from the groups may be C-linked or N-linked at possible positions. For example, the group derived from pyrrole may be pyrrole-1-yl (N-linked) or pyrrole-3-yl (C-linked). In addition, the group derived from imidazole may be imidazol-1-yl (N-linked) or imidazol-3-yl (C-linked). An example of a heterocyclic group in which two ring carbon atoms are substituted with oxo (═O) is 1,1-dioxo-thiomorpholinyl.
[143] As used herein, the term “pharmaceutically acceptable salts” includes salts of acidic or basic groups that may be present in the compound of Formula 1 unless otherwise indicated. Compounds of formula (I) that are basic in nature are capable of forming a wide range of salts of various inorganic and organic acids. Acids that can be used to prepare pharmaceutically acceptable acid addition salts of such basic compounds of formula (1) are non-toxic acid addition salts, ie salts containing pharmacologically acceptable anions such as acetate, benzenesulfonate, benzoate , Bicarbonate, bisulfate, bitartrate, borate, bromide, calcium edetate, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, ecylate , Ethyl succinate, fumarate, gluceptate, gluconate, glutamate, glycollylasanylate, hexylsorbinate, hydravamin, hydrobromide, hydrochloride, iodide, isothionate, lactate, Lactobionate, laurate, maleate, maleate, mandelate, Mesylate, methylsulfate, kate free, naphsylate, nitrate, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, phosphate / diphosphate, polygalacturonate, salicylate , Acids that form stearates, subacetates, succinates, tanates, tartrates, the octolates, tosylates, triethiodes and valerate salts. Since a single compound of the present invention may comprise one or more acidic or basic residues, the compounds of the present invention may include mono-, di- or tri-salts in a single compound.
[144] Compounds of the invention that are acidic in nature can form base salts with various pharmacologically acceptable cations. Examples of such salts include alkali metal or alkaline earth metal salts, and especially the calcium salts, magnesium salts, sodium salts and potassium salts of the compounds of the invention.
[145] In compounds of formula (1) in which terms such as (CR ¹ R ² ) _q or (CR ¹ R ² ) _t are used, R ¹ and R ² may vary with one or more repeating q or t, respectively. For example, when q or t is 2, (CR ¹ R ² ) _q or (CR ¹ R ² ) _t is -CH ₂ CH ₂ -or -CH (CH ₃ ) C (CH ₂ CH ₃ ) (CH ₂ CH ₂ CH ₃ )-, or any number of similar moieties that fall within the definition of R ¹ and R ² . Also, as described above, any substituents include a halogeno, SO or SO ₂ group, or a CH ₃ (methyl), CH ₂ (methylene) or CH (methine) group not bonded to an N, O or S atom. Optionally has a substituent in the group selected from hydroxy, C ₁ -C ₄ alkoxy and —NR ¹ R ² .
[146] In the compound of Formula 1, R ⁴ is-(CR ¹ R ² ) _t -CR ¹ R ¹¹ R ¹² , R ¹² group is preferably bonded via a carbon atom when the monocyclic ring, carbon atom when the bicyclic ring Or via nitrogen.
[147] Certain compounds of formula (1) may have asymmetric centers and therefore exist in different mirror images. All optical isomers and stereoisomers of Formula 1, and mixtures thereof, are considered to be within the scope of the present invention. In the compounds of formula 1, the present invention encompasses the use of racemates thereof, one or more enantiomeric forms, one or more diastereomeric forms or mixtures thereof. Compounds of formula 1 may also exist as tautomers. The present invention relates to the use of all such tautomers and mixtures thereof.
[148] The present invention also includes isotopically-labeled compounds, which are identical to those recited in Formula 1, but wherein one or more atoms are typically substituted by atoms having an atomic weight or number of atoms different from the originally known atomic weight or number of atoms. Examples of isotopes that may be incorporated into the compounds of the present invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine and chlorine, such as ² H, ³ H, ¹³ C, ¹⁴ C, ¹⁵ N, ¹⁸ O. , ¹⁷ O, ³¹ P, ³² P, ³⁵ S, ¹⁸ F and ³⁶ Cl. Compounds of the present invention, prodrugs thereof, and pharmaceutically acceptable salts of these compounds or prodrugs that contain such isotopes and / or other isotopes of other atoms are included within the scope of the present invention. Certain isotopically-labelled compounds of the invention, such as those incorporating radioisotopes such as ³ H and ¹⁴ C, are useful for drug and / or substrate tissue distribution analysis. Tritium, ie ³ H and carbon-14, ie ¹⁴ C isotopes are particularly preferred in view of ease of preparation and detection. In addition, substitution with heavier isotopes, such as deuterium, i.e. ² H, results in greater metabolic stability, such as increased in vivo half-life or reduced dose requirements, which may provide certain therapeutic benefits. May be preferred. Isotope-labeled compounds of formula (1) and prodrugs thereof of the present invention are generally the following schemes and / or examples and preparations by substituting non-isotopically-labeled reagents with readily available isotopically-labeled reagents. It can be prepared by performing the procedure disclosed in the examples.
[149]
[150]
[1] The present invention relates to novel morbid derivatives useful for the treatment of abnormal cell growth such as cancer in mammals. The invention also relates to methods of using said compounds in the treatment of abnormal cell growth in mammals, in particular humans, and to pharmaceutical compositions containing said compounds.
[151] General synthetic methods which may be referred to for the preparation of the compounds of the present invention are described in U.S. Patent No. 5,747,498 (registered May 5, 1998), U.S. Patent Application 08/953078 (filed Oct. 17, 1997). WO 98/02434 published 22 January 1998, WO 98/02438 published 22 January 1998, WO 96/40142 published 19 December 1996, WO 96/09294 published March 6, 1996, WO 97/03069 published 30 January 1997, WO 95/19774 published 27 July 1995 and WO 97 / 13771 (published April 17, 1997). The above patents and patent applications are incorporated herein by reference in their entirety. Certain starting materials may be prepared according to methods familiar to those skilled in the art, and certain modified synthesis methods may also be performed according to methods familiar to those skilled in the art. Standard preparation of 6-iodoquinazolinone is described by Stevenson, TM, Kazmierczak, F., Leonard, NJ, J. Org. Chem. 1986, 51, 5, p. 616 ". The palladium-catalyzed boronic acid coupling agent is described in Miyaura, N., Yanagi, T., Suzuki, A., "Syn. Comm. 1981, 11, 7, p. 513". It is described in Palladium-catalyzed Heck coupling agents are described in Heck et al., &Quot; Organic Reactions, 1982, 27, 345 " or Cabri et al., &Quot; Acc. Chem. Res. 1995, 28, 2 " It is described in Examples of palladium-catalyzed coupling of terminal alkynes to aryl halides are described by Castro et al., J. Org. Chem. 1963, 28, 3136 or Sonogashira et al. Synthesis, 1977, 777. "Is presented. For the formation of alkyl and cycloalkylzinc reagents, those skilled in the art have reviewed Rieke, RD, Hanson, MV, Brown, JD, and Niu, QJ. . Chem., 1996, 61, 8, p. 2726 ". Azetidinyl zinc chemistry can be carried out using the method in Bilotte, S., "Synlett, 1998, 379". Terminal alkyn synthesis is described by Colvin, E.W.J. et al., Chem. Soc. Perkin Trans. I, 1977, 869; Gilbert, J. C. et al., J. Org. Chem., 47, 10, 1982; Hauske, J.R. et al., Tet. Lett., 33, 26, 1992, 3715 "; Ohira, S. et al., J. Chem, Soc. Chem. Commun., 9, 1992, 721; Trost, B.M., " J. Amer, Chem, Soc., 119, 4, 1997, 698 "; Or using appropriately substituted / protected aldehydes, as described in Marshall, J.A. et al., &Quot; J. Org. Chem., 62, 13, 1997, 4313 ".
[152] Alternatively, terminal alkyne can be prepared by a two-step procedure. First, a lithium anion of TMS (trimethylsilyl) acetylene is added to a properly substituted / protected ketone or aldehyde, as in Nakatani, K. et al., &Quot; Tetrahedron, 49, 9, 1993, 1901 " By base, as in Malacria, M. "Tetrahedron, 33, 1977, 2813" or White, JD et al. "Tet. Lett., 31, 1, 1990, 59". Subsequent deprotection can be used to isolate the intermediate terminal alkyl. Aryl amines such as phenoxyaniline, benzyloxyaniline, phenylsulfonylindole, benzylindole or benzylindazole can be prepared by reducing the corresponding nitro intermediates. Aromatic nitro groups are described in Brown and Nelson, N. A., J. Org. Chem. 1954, p. 5149; Yuste, R., Saldana, M., Wals, F., Tet. Lett. 1982, 23, 2, p. 147; Or by the method outlined in WO 96/09294 mentioned above. Nitro-substituted N1-phenylsulfonylindole / indazoles are described in Sundberg, R.J., Bloom, J.D., J. Org. Chem. 1980, 45, 17, p. 3382 "; Ottoni, O. et al., Tetrahedron, 1998, 54, 13915; Or by Boger, Dale L. et al., J. Org. Chem. 55, 4, 1990, 1379. Substituted nitro N1-benzylindoles / indazoles are described by Makosza, M., Owczarczyk, Z. "J. Org. Chem., 54, 21, 1989, 5094"; Adebayo, Adelaide T.O.M. et al., J. Chem. Soc. Perkin Trans. 1, 1989, 1415; Or by the process in WO 98/02434 mentioned above. Benzyloxynitrobenzene intermediates may be prepared by the process in WO 98/02434 mentioned above. Alternatively, arylmethoxy or aryloxy nitrobenzene derivatives are described in Dinsmore, C.J. et al., "Bioorg. Med. Chem. Lett., 7, 10, 1997, 1345"; Louy, A. et al., "Synth. Commun., 20, 18, 1990, 2855"; Or by nucleophilic substitution of a halide with an appropriate alcohol from a halo nitrobenzene precursor as described in Brunelle, D.J., "Tet. Lett., 25, 32, 1984, 3383".
[153] Starting materials for which the synthesis is not specifically described are commercially available or can be prepared using methods well known to those skilled in the art.
[154] In each reaction discussed or illustrated in the above schemes, the pressure is not critical unless otherwise noted. Pressures of about 0.5 to about 5 atmospheres are generally acceptable, with ambient pressure being preferred for convenience.
[155] If the compound of the formula HNR ¹ R ³ is an optionally substituted indole or indolin moiety, such compound may be prepared according to one or more methods known to those skilled in the art. Such methods are described in PCT International Patent Application Publication No. WO 95/23141, and by WC Sumpter and Miller ("Heterocyclic Compounds with Indole and Carbazole Systems," in volume 8 of "The Chemistry of Heterocyclic Compounds). , Interscience Publishers Inc., New York (1954). Optional substituents may be included as appropriate before or after the coupling step shown in Scheme 1. Prior to the coupling step, the primary and secondary amino residues (other than the amines of the formula HNR ¹ R ³ ) are preferably protected using nitrogen protecting groups known to those skilled in the art. Such protecting groups and their use are described in TW Greene and PGM Wuts, "Protective Groups in Organic Synthesis," Second Edition, John Wiley & Sons, New York, 1991.
[156] In Scheme 1, the compound of Formula 1 is a compound of Formula 2 wherein X, A and R ⁴ are as defined above, Z is a leaving group, such as a substituted phenoxy derivative (such as halo, cyano , Nitro and / or C ₁ -C ₆ alkyl group) or chloro) may be added to anhydrous solvents, especially DMF (N, N-dimethylformamide), DME ( Ethylene glycol dimethyl ether), DCE (dichloroethane), t-butanol and phenol, or a mixture of the above solvents can be prepared by coupling with an amine of formula 3 wherein R ¹ and R ³ are as defined above. have. Compounds of formula 3 can be prepared by methods known to those skilled in the art, such as reduction of nitriles, reduction of imines or enamines, reduction of oximes, primary and secondary amides, reduction of nitro groups, or R ¹ NH ₂ and It can be prepared by the reductive amination of R ³ CH (O) or R ³ NH ₂ and R ¹ CH (O). The compound of formula 2 is a compound of formula 4 mentioned in Scheme 2 wherein Z ¹ is an activation group such as bromo, iodo, -N ₂ , or -OTF (ie -OSO ₂ CF ₃ ), or an activation group Is a precursor, such as NO ₂ , NH ₂ or OH), by treating with a coupling partner such as terminal alkyne, terminal alkene, vinyl halide, vinyl stanan, vinylborane, alkyl borane, or alkyl or alkenyl zinc reagent. Can be.
[157] Alternatively, the compound of formula 1 may be prepared according to the synthesis outlined in Scheme 2. In Scheme 2, the compound of formula 8 wherein X is NH is a compound of formula 9 according to one or more procedures described in the aforementioned WO 95/19774 wherein A and Z ¹ are as defined above , Z ³ can be prepared from NH ₂ , C ₁ -C ₆ alkoxy or OH, and the compound of formula 8, wherein X is CH, according to the procedure described in WO 95/19774 mentioned above. Can be prepared from a compound of formula 10 wherein A and Z ¹ are as defined above. The compound of formula 8 may be prepared by treating the starting compound with a chlorinating agent (e.g. POCl ₃ or ClC (O) C (O) Cl / DMF) in a halogenated solvent at about 60 to 150 C for about 2 to 24 hours. Can be converted to a compound. The compound of formula 7 may be prepared by treating the starting compound with an appropriate metal phenoxide (eg sodium phenolate) in a solvent (eg DMF or phenol) at about 0-100 ° C. for about 2 to 24 hours, wherein Z is a substituted phenoxy derivative). The compound of formula 6 may be reacted with a coupling partner such as terminal alkyne, terminal alkene, vinyl stanan, vinyl borane, alkyl borane, or an alkyl or alkenyl zinc reagent to afford the compound of formula 2. The compound of formula 2 may then be modified into a compound of formula 1 by coupling with an amine of formula 3. Alternatively, the compound of formula 1 is prepared by reacting terminal alkyne, terminal alkene, vinyl halide, vinyl stanan, vinyl borane, alkyl borane, or alkyl or alkenyl zinc reagent with the compound of formula 7 to obtain an intermediate of formula 11 Can be. Intermediate 11 can subsequently be coupled with an amine of formula 3 to yield a compound of formula 1. Another method for the synthesis of derivatives of Formula 1 includes coupling chloroquinazolin 7 with amine 3 and then intermediate 5 with terminal alkyne, terminal alkene, vinyl halide, vinyl stanan, vinyl borane, alkyl borane, or alkyl Or coupling with an alkenyl zinc reagent.
[158] The compounds of the present invention may have asymmetric carbon atoms. Diastereomeric mixtures can be separated into individual diastereomers on the basis of physicochemical differences by methods known in the art, such as chromatography or fractional crystallization. Enantiomers convert enantiomeric mixtures into diastereomeric mixtures by reaction with appropriate optically active compounds (e.g. alcohols), separate the diastereomers, and convert the individual diastereomers into the corresponding pure enantiomers. Can be separated (eg by hydrolysis). All such isomers, including diastereomeric mixtures and pure enantiomers, are contemplated as part of the present invention.
[159] Compounds of formula (I), which are inherently basic, can use a variety of inorganic and organic acids to form a wide variety of different salts. Although such salts may be pharmaceutically acceptable for animal administration, in practical terms, the compounds of Formula 1 are initially isolated from the reaction mixture as pharmaceutically unacceptable salts and then treated with alkaline reagents and subsequently It is preferred to simply convert the free base to a free base compound by converting the free base into a pharmaceutically acceptable acid addition salt. Acid addition salts of the base compounds of the invention are readily prepared by treating the base compounds with substantially equal amounts of selected mineral or organic acids in an aqueous solvent medium or a suitable organic solvent such as methanol or ethanol. By evaporating the solvent carefully, the desired solid salt is easily obtained. The desired acid salt can also be precipitated from a solution of the free base in an organic solvent by adding the appropriate mineral or organic acid to the solution.
[160] These naturally acidic compounds of formula (1) can use various pharmacologically acceptable cations to form base salts. Examples of such salts include alkali metal or alkaline earth metal salts and in particular sodium and potassium salts. All of these salts are prepared by conventional methods. The chemical base used as reagent in the preparation of the pharmaceutically acceptable base salt of the present invention is the formation of non-toxic base salts using the acidic compound of formula (1). Such non-toxic base salts include those derived from pharmacologically acceptable cations such as sodium, potassium, calcium and magnesium. These salts can be readily prepared by treating the corresponding acidic compounds with an aqueous solution containing the desired pharmacologically acceptable cations and then evaporating to dryness the resulting solution, preferably under reduced pressure. Alternatively, they can also be prepared by mixing a lower alkanolic solution of an acidic compound with the desired alkali metal alkoxide and then evaporating to dryness the resulting solution in the same manner as before. In both cases, stoichiometric amounts of reagents are preferably used to ensure complete reaction and maximum yield of the desired final product. Since a single compound of the present invention may comprise one or more acidic or basic residues, the compounds of the present invention may include mono-, di- or tri-salts in a single compound.
[161] Compounds of the present invention are potent inhibitors of the erbB family of tumorigenic and protoongenic protein tyrosine kinases such as epidermal growth factor receptor (EGFR), erbB2, HER3 or HER4, thereby all of which are antiproliferative in mammals, especially humans It is suitable for use in therapy as an agent (eg anticancer agent). In particular, the compounds of the present invention can be used in various human hyperproliferative diseases such as liver, kidney, bladder, breast, stomach, ovary, colon, prostate, interest, lungs, vagina, thyroid, liver carcinoma, sarcoma, glioblastomas, head And chronic and benign tumors of the neck and other hyperplastic diseases such as benign hyperplasia of the skin (eg psoriasis) and benign hyperplasia of the prostate (eg BPH). It is also expected that the compounds of the present invention may have activity against leukemias and lymphoid malignancies.
[162] The compounds of the present invention may also be useful for the treatment of additional diseases associated with aberrant expression ligand / receptor interactions or activities, or signaling cases involving various protein tyrosine kinases. Such diseases include diseases of the nervous system, glial, astrocytes, hypothalamus, and other glands, macrophages, epithelial cells, which are associated with aberrant function, expression, activation or signaling of erbB tyrosine kinase. Diseases of stromal and blastocoelic properties may be included. In addition, the compounds of the invention may have therapeutic utility in inflammatory, angiogenic and immune diseases associated with identified or unidentified tyrosine kinases that are inhibited by the compounds of the invention.
[163] The ex vivo activity of the compound of formula 1 can be measured by the following procedure.
[164] The c-erbB2 kinase assay is similar to that already described in Schrang et al., Anal. Biochem. 211, 1993, p233-239. Nunc MaxiSorp 96-well plates were prepared with 0.25 mg / ml of Poly (Glu, Tyr) 4: 1 (PGT) (Sigma Chemical Co., St. Louis, MO) in PBS (phosphate buffered saline). 100 ml per well is used to incubate overnight at 37 ℃ coating. Excess PGT is removed by aspiration and plates are washed three times with wash buffer (0.1% Tween 20 in PBS). Kinase reactions were performed at 50 mM containing 125 mM sodium chloride, 10 mM magnesium chloride, 0.1 mM sodium orthovanadate, 1 mM ATP, 0.48 mg / ml (24 ng / well) c-erbB2 intercellular domain. In 50 ml of HEPES pH 7.5. The intercellular domain of erbB2 tyrosine kinase (amino acids 674-1255) is expressed as GST fusion protein in Baculovirus and purified by binding to and eluting glutathione-coated beads. The compound in DMSO (dimethylsulfoxide) is added so that the final DMSO concentration is about 2.5%. Phosphorylation is initiated by the addition of ATP (adenosine triphosphate) and run for 6 minutes at room temperature with constant stirring. The kinase reaction is terminated by aspirating the reaction mixture and subsequently washing with wash buffer (see above). Phosphorylated PGT was HRP-conjugated PY54 (Oncogin Science Inn, Uniondale, NY) diluted at 0.2 mg / ml in blocking buffer (3% BSA in PBS and 0.05% Tween 20). Oncogene Science Inc.) Antiphosphotyrosine antibodies are measured by incubating for 25 minutes using 50 ml per well. Antibodies are removed by aspiration and plates are washed four times with wash buffer. Colorimetric signals were developed by adding 50 ml of TMB Microwell Peroxidase Substrate (Kirkegaard and Perry, Gaitsburg, MD) per well and 50 ml of 0.09 M per well Termination is performed by adding sulfuric acid. Phosphotyrosine is assessed by measuring absorption at 450 nm. The signal from the control is typically an absorption unit of 0.6 to 1.2 with essentially no background in wells without PGT substrate and is proportional to incubation time for 10 minutes. Inhibitors are identified by reduction of the signal to wells without inhibitors and IC ₅₀ values corresponding to the concentration of compound required for 50% inhibition are determined.
[165] In vivo activity of the compound of formula 1 can be measured by the amount of inhibition of tumor growth by the test compound compared to the control. Tumor Induction Relationships in Development of Transplantable Cancers of the Colon in Mice for Chemotherapy Assays, with a Note on Carcinogen Structure ", Cancer Res., 35, Corbett, TH, et al. , 2434-2439 (1975) and Corbett et al., "A Mouse Colon-tumor Model ofr Experimental Therapy", Cancer Chemother. Rep. (Part 2) ", 5, 169-186 (1975), and a slightly modified method. Tumors were measured in 1-5 million log phase suspended in 0.1 ml RPMI 1640 medium. It is injected into the left flank by subcutaneous (sc) injection.After enough time has passed to detect the tumor, it becomes detectable (diameter of 100 to 150 mm / 5/5 to 6 mm) and then the test animal ( athymic) female mice) were tested compound (in combination at a concentration of 10-15 mg / ml in 5 Gelucire) by the intraperitoneal (ip) or oral (po) route once or twice daily for 7 to 10 consecutive days. To measure antitumor effects, tumors are measured in millimeters with Vernier calipers, and tumor size is determined by Geran, RI et al., Protocols for Screening Chemical Agents and Natural. Products Against Animal tumors and Other Biological Systems ", third Edition, Cancer Chemother. Rep., 3, 1-104 (1972); La is calculated using the following equation:
[166]
[167] The results are expressed as percent inhibition according to Equation 2:
[168]
[169] The tumor-grafted flanks show a reproducible dose / response effect for various chemotherapeutic agents, and the (tumor diameter) measurement method is variable for tumor growth rate.
[170] Administration of a compound of the invention (hereafter “active compound”) can deliver the compound to the active site, which can be administered by any method. These methods include oral route, duodenal route, parenteral injection (including intravenous, subcutaneous, intramuscular, endovascular or infusion), topical administration and rectal administration.
[171] The amount of active compound administered will depend upon the subject being treated, the neutrality of the disease or condition, the rate of administration, the distribution of the compound and the discretion of the attending physician. However, the effective dose is about 0.001 to about 100 mg / kg body weight per day, preferably about 1 to about 35 mg / kg body weight daily, in single or divided doses. In a 70 kg human, it is about 0.05 to about 7 g / day, preferably about 0.2 to about 2.5 g / day. In some cases, dosage levels below the lower limit of the aforementioned ranges may be above the appropriate levels, while in other cases large dosages may still be used that do not cause any adverse side effects, although such large dosages should be administered throughout the day first. For several small doses.
[172] The active compound may be administered alone or as one or more other antitumor agents, such as mitosis inhibitors (eg vinblastine); Alkylating agents (eg, cis-platin, carboplatin and cyclophosphamide); Antimetabolites (eg 5-fluorouracil, cytosine arabinoside and hydroxyurea) or one of the preferred antimetabolites disclosed in, eg, European Patent Application 239362, such as N- (5- [N- (3 , 4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl) -N-methylamino] -2-tenoyl) -L-glutamic acid; Growth factor inhibitors; Cell cycle inhibitors; Intercalating antibiotics (eg, adriamycin and breomycin); Enzymes such as interferon; And anti-hormonal agents such as antiestrogens, such as Nolvadex® (tamoxifen), or for example Casodex® Casodex (4′-cyano-3- (4-fluorophenylsulfonyl) -2 -Hydroxy-2-methyl-3 '-(trifluoromethyl) propionanilide). Such interlinked therapy may be achieved by simultaneous, continuous or separate administration of the individual components.
[173] Pharmaceutical compositions include, for example, oral administration such as tablets, capsules, pills, powders, sustained release preparations, solutions, suspensions; Parenteral injection such as sterile solutions, suspensions or emulsions; Topical administration such as ointments or creams; Or in a form suitable for rectal administration such as suppositories. The pharmaceutical composition may be in unit dosage forms suitable for single administration of precise administration. The pharmaceutical composition will comprise as active ingredient a compound according to the invention and a conventional pharmaceutical carrier or excipient. It may also include other medical or pharmaceutical agents, carriers, adjuvants and the like.
[174] Examples of parenteral dosage forms include solutions or suspensions of the active compounds in sterile aqueous solutions, such as aqueous propylene glycol or textrose solutions. Such dosage forms can be appropriately buffered if necessary.
[175] Suitable pharmaceutical carriers include inert diluents or fillers, water and various organic solvents. The pharmaceutical composition may, if desired, contain additional ingredients such as flavoring agents, binders, excipients and the like. Thus, for oral administration, tablets containing various excipients such as citric acid can be used with various disintegrants such as starch, alginic acid and certain complex silicates, and binders such as sucrose, gelatin and acacia. In addition, lubricants such as magnesium stearate, sodium lauryl sulfate and talc are often useful for tableting purposes. Solid compositions of a similar type may also be used in soft and hard filled gelatin capsules. Preferred materials for this include lactose or lactose, and high molecular weight polyethylene glycols. Where aqueous suspensions or elixirs are required for oral administration, the active compounds therein may be sweetening agents, flavoring agents, coloring substances or dyes, if necessary emulsifiers or suspending agents, and diluents (e.g. water, ethanol, propylene glycol, glycerin or their Mixtures).
[176] Methods for preparing various pharmaceutical compositions having specific amounts of active compounds will be known or will be apparent to those skilled in the art. See, eg, Remington's Pharmaceutical Sciences , Mack Publishing Company, Easter, Pa., 15th Edition (1975).
[177] The examples and preparations set forth below further illustrate and illustrate the compounds of the present invention and methods of preparing such compounds. It is believed that the scope of the present invention is not limited in any way by the scope of the following examples and preparations. In the examples below, molecules with a single chiral center are present as racemic mixtures unless otherwise noted. Molecules having two or more chiral centers exist as racemic mixtures of diastereomers unless otherwise stated. Single enantiomers / diastereomers may be obtained by methods known to those skilled in the art.
[178] When HPLC chromatography is mentioned in the preparations and examples below, the general conditions used are as follows unless otherwise stated. The column used is a Jorbox ™ ZXBA 18 column (manufactured by Hewlett Packard) with a distance of 150 mm and an internal diameter of 4.6 mm. Samples are run on a Hewlett Packard-1100 system. The gradient solvent method uses 100% acetonitrile from 100% ammonium acetate / acetic acid buffer (0.2 M) over 10 minutes. The system then proceeds on a wash cycle using 100% acetonitrile for 1.5 minutes and then on 100% buffered solution for 3 minutes. The flow rate during this period is 3 ml / min.
[179] In the examples and preparations below, "Et" refers to ethyl, "Ac" refers to acetyl, "Me" refers to methyl, and "Bu" refers to butyl.
[180] Preparation Example of 3-methyl-4-phenoxynitrobenzene
[181] Sodium hydride (95% dry powder) (83.62 g, 3.31 moles, 1.3 equiv) was purged under a nitrogen atmosphere in a clean-dried 12 liter, equipped with a condenser, a dropping funnel, a mechanical stirrer and two nitrogen-outlet bubblers. Four-necked flasks were filled (note: sodium hydride is pyrophoric and should be avoided in contact with water or moisture). After the reaction flask was cooled to 0 ° C. (ice bath), anhydrous DMF (1280 mL) was added carefully using a dropping funnel. The reaction mixture was stirred for 30 min at 0 ° C., then a solution of phenol (263.5 g, 2.8 moles, 1.1 equiv) in anhydrous DMF (1280 mL) was added over 2 hours using a dropping funnel (Note: exothermic , Heated hydrogen release). After the addition was complete, the reaction mixture was stirred at 0 ° C. for 40 minutes (the reaction mixture turned into a white slurry) and then 3-methyl-4-fluoronitrobenzene in anhydrous DMF (dimethylformamide) (1280 mL) The solution (390.0 g, 2.51 moles, 1.0 equiv) was added dropwise over 1 hour. The reaction mixture was slowly warmed to room temperature and stirred at room temperature for 15 to 22 hours (TLC, 2% ethyl acetate in hexane) until all starting material was converted to phenoxynitrotoluene (dark brown viscous solution). Again, the reaction mixture was cooled to 0 ° C. (ice bath) and then quenched carefully with cold water (5000 mL) over 2 hours (Note: exothermic, hydrogen evolution; first add 100 mL of water over 90 minutes). . The reaction mixture was stirred for 1 hour and then transferred to two 50 L-carboys each containing 40 L of water. The contents were stirred and left at room temperature for 24 hours to give phenoxynitrotoluene as a yellow solid. The yellow solid was filtered off, washed with excess water and air dried to afford 3-methyl-4-phenoxynitrobenzene (552 g, 96% yield). Crude 3-methyl-4-phenoxynitrobenzene was found to be pure by ¹ H and ¹³ C NMR spectrometers and used as such for the following reactions: melting point 51-52 ° C .; FT-IR (cm ⁻¹ ): 1582, 1509, 1480, 1339, 1242, 1204, 1091 and 796; ¹ H NMR (300 MHz, CDCl ₃ ) δ 2.41 (s, 3 H), 6.78 (d, 1 H, J = 8.7 Hz), 7.02-7.08 (m, 2H), 7.19-7.29 (m, 1 H ), 7.38-7.46 (m, 2H), 7.99 (dd, 1H, J = 9.15 Hz, 2.7 Hz); ¹³ C NMR (75.45 MHz, CDCl ₃ ) 16.22, 115.93, 119.11, 123.17, 124.9, 126.79, 129.53, 130.28, 142.66, 155.44 and 161.4.
[182] Preparation Example of 3-methyl-4-phenoxyaniline hydrochloride
[183] To a solution of 3-methyl-4-fluoronitrobenzene (2) in methanol (5 L) (548 g, 2.39 mol, 1.0 equiv) at 10% Pd / C (100 g, 50% humidity, 46.98 mmol, 0.02 Equivalent)) was added. The reaction mixture was then stirred under hydrogen atmosphere (60-80 psi) for 15-16 hours at room temperature in a 2-gallon Par hydrogen hydrogenator. The progress of the reaction was monitored by TLC (50% ethyl acetate in hexane, sm Rf = 0.69, pr Rf = 0.47, UV clockable). The reaction mixture was then filtered through Celite and the solid was washed with excess methanol. The filtrate was concentrated under reduced pressure to afford 3-methyl-4-phenoxyaniline (451.0 g, 95%) as a light brown viscous liquid. 3-methyl-4-phenoxyaniline was found to be pure by ¹ H and ¹³ C NMR spectrometers and used as such for the next reaction.
[184] In a 3-methyl-4-phenoxyaniline solution (451.0 g, 2.26 mol, 1.0 equiv) cooled by stirring (to 0 ° C.) in anhydrous ether (12 L), when all starting materials are converted to the aniline hydrochloride salt Anhydrous HCl gas was generated for 40 to 90 minutes. The off-white solid was filtered, washed with ether and dried in a vacuum oven at 60 ° C. for 6 hours to give 3-methyl-4-phenoxyaniline hydrochloride (511.8 g, 96%): melting point 173 to 174 ° C. ; FT-IR (cm ⁻¹ ): 3058, 3019, 2840, 2573, 1485, 1253, 1223 and 691; ¹ H NMR (300 MHz, CDCl ₃ ) δ 2.22 (s, 3 H), 6.81-6.9 (m, 3 H), 7.04-7.11 (m, 1 H), 7.25-7.37 (m, 3 H), 7.43 (d, 1H, J = 2.4 Hz), 10.45 (s, 3H); ¹³ C NMR (75.45 MHz, CDCl ₃ ) 16.03, 118.01, 119.9, 122.12, 123.35, 124.78, 126.13, 129.93, 131.89, 155.5 and 156.96; APCI (negative FAB) 200.3 (100%); Analytical Calcd for C ₁₃ H ₁₄ CINO: C, 66.24; H, 5.99; N, 5.94. Found: C, 60.05; H, 6.01; N, 5.98.
[185] Examples of other amines produced by this method are as follows:
[186] 3-chloro-4-phenoxy-phenylamine,
[187] 3-methoxy-4-phenoxy-phenylamine,
[188] 4-phenoxy-3-trifluoromethyl-phenylamine,
[189] 3-fluoro-4-phenoxy-phenylamine,
[190] 5-amino-2-phenoxy-benzonitrile,
[191] 4- (2-methoxy-phenoxy) -3-methyl-phenylamine,
[192] 4- (3-methoxy-phenoxy) -3-methyl-phenylamine,
[193] 4- (4-methoxy-phenoxy) -3-methyl-phenylamine,
[194] 4- (2-fluoro-phenoxy) -3-methyl-phenylamine,
[195] 4- (3-fluoro-phenoxy) -3-methyl-phenylamine,
[196] 4- (4-fluoro-phenoxy) -3-methyl-phenylamine,
[197] 4- (2-methyl-phenoxy) -3-methyl-phenylamine,
[198] 4- (3-methyl-phenoxy) -3-methyl-phenylamine,
[199] 4- (4-methyl-phenoxy) -3-methyl-phenylamine,
[200] 4- (2,6-difluoro-phenoxy) -3-methyl-phenylamine,
[201] 3,5-dichloro-4-phenoxy-phenylamine,
[202] 3-methyl-4-phenylsulfanyl-phenylamine,
[203] 4-phenylsulfanyl-phenylamine,
[204] 4-cyclohexyloxy-3-methyl-phenylamine,
[205] 4-cyclopentyloxy-3-methyl-phenylamine,
[206] 4-cyclobutyloxy-3-methyl-phenylamine,
[207] 2-fluoro-4-phenoxyamine,
[208] 4-fluoro-2-phenoxyamine,
[209] 3-bromo-4-phenoxy-phenylamine,
[210] 4- (2-chloro-phenoxy) -3-methyl-phenylamine,
[211] 4- (2-methoxy-phenoxy) -3-methyl-phenylamine,
[212] 4- (2-ethyl-phenoxy) -3-methyl-phenylamine,
[213] 4- (2-trifluoromethyl-phenoxy) -3-methyl-phenylamine,
[214] 1- (5-amino-2-phenoxy-phenyl) -ethanone; And
[215] (+/-)-4-benzenesulfinyl-3-methyl-phenylamine, (+/-)-4-benzenesulfinyl-phenylamine, 4-benzenesulfonyl-3-methyl-phenylamine and 4-benzene Sulfonyl-phenylamine (these are prepared from 3-methyl-4-phenylsulfanyl-phenylamine and 4-phenylsulfanyl-phenylamine by oxidation methods known to those skilled in the art).
[216] 3-ethyl-4-phenoxy-phenylamine
[217] To a 1- (5-amino-5-phenoxy-phenyl) -ethanone solution (0.5 g, 2.20 mmol) in THF (15 mL) sodium borohydride (0.4 g, 10.5 mmol) and AlCl ₃ (anhydride) under nitrogen (0.803 g, 6.02 mmol) was added. The resulting reaction mixture was heated at reflux for 4 hours. The mixture was then cooled and ice water was added. The resulting mixture was extracted with EtOAc and dried over Na ₂ SO ₄ . Solvent was removed to give a brownish residue which was chromatographed with 4: 1 hexanes / EtOAc to give 3-ethyl-4-phenoxy-phenylamine product (15 mg, 10%).
[218] 3-hydroxy-4-phenoxy-phenylamine
[219] 3-methoxy-4-phenoxynitrobenzene (2 g, 8.15 mmol) was treated with 48% HBr (20 mL) and HOAc (20 mL). After the reaction mixture was heated to 110 ° C. for 24 h, the reaction mixture was poured into ice and extracted with EtOAc, the organic layer was washed with brine and dried over Na ₂ SO ₄ . The solvent was removed to give a brownish residue 5-nitro-2-phenoxy-phenol, which was used in the next step without further purification. (Almost quantitative yield). ¹ H NMR (CDCl ₃ ): δ 7.91 (d, 1 H, 2.7 Hz), 7.72 (dd, 1 H, J 1 = 8.8 Hz, J 2 = 2.4 Hz), 7.43 (t, 2 H, J = 7.9 Hz) , 7.28 (d, 1H, 7.9 Hz), 7.10 (d, 1H, J = 8.3 Hz), 6.78 (d, 2H, J = 8.9 Hz).
[220] Ethoxy-4-phenoxy-phenylamine
[221] To a 5-nitro-2-phenoxy-phenol solution (500 mg, 2.16 mmol) in acetone (20 mL) was added bromoethane (0.353 g, 3.26 mmol) and potassium carbonate (0.447 g, 3.26 mmol). The resulting reaction mixture was stirred for 2 hours at room temperature, then the reaction was heated to 50 ° C. for 4 hours. Water was added and the aqueous layer was extracted with EtOAc (3 times, 30 mL) and the organic layer was washed with brine and dried over Na ₂ S0 ₄ . Solvent was removed to give 3-ethoxy-4-phenoxy-nitrobenzene (0.3 g, 53%). The product was hydrogenated on Pd-C (%) in methanol to afford 3-ethoxy-4-phenoxy-phenylamine (0.1 g, 38%). M / z, 230.0, ¹ H NMR (CDCl ₃ ): δ 7.91 (d, 1 H, 2.7 Hz), 7.72 (dd, 1 H, J 1 = 8.8 Hz, J 2 = 2.4 Hz), 7.43 (about t, 2 H, J = 7.9 Hz), 7.28 (d, 1 H, 7.9 Hz), 7.10 (d, 1 H, J = 8.3 Hz), 6.78 (d, 2 H, J = 8.9 Hz), 4.17 (dd, 2 H, J1 = 13.9 Hz, J2 = 7.1 Hz), 1.42 (t, 3H, J = 7.1 Hz).
[222] 3-isopropoxy-4-phenoxy-phenylamine was also prepared by the above alkylation protocol.
[223] 3-phenyl-1H-indazol-6-ylamine
[224] Anhydrous hydrazine (120 mg) was added to a 2-chloro-5-nitro-benzophenone solution (1.0 g) in THF (tetrahydrofuran) (15 mL). The resulting reaction mixture was stirred continuously at room temperature for 2-4 hours. The solvent was removed in vacuo and the residue was dissolved in EtOAc, washed with water and brine and dried over Na ₂ SO ₄ . Solvent was removed to give product 6-nitro-3-phenyl-1H-indazole (5) (0.8 g, 88%). 6-nitro-3-phenyl-1H-indazole was hydrogenated over H ₂ / Pd to give 0.5 g (71.5%) of 3-phenyl-1H-indazol-6-ylamine. M / z, 210.0. ¹ H NMR (CD ₃ OD): 7.86 (d, 2H, J = 7.9 Hz), 7.47 (t, J = 8.1 Hz), 7.35 (t, 3H, J = 8.7 Hz), 7.01 (d, 1 H, J = 8.7 Hz).
[225] General procedure for the addition of 1-rithio-1-trimethylsilylacetylene to carbonyl
[226] A cold (-78 ° C.) stirred (trimethylsilyl) acetylene solution (1.2 equiv) in anhydrous THF was treated with nBuLi (1.2 equiv) under nitrogen (for triethylsilyl, BOC-protected amino aldehyde containing free NH). The amount of acetylene and n-BuLi is double). The colorless solution was stirred for 30-40 minutes and then carbonyl compound (1.0 equiv) was added in anhydrous THF. The reaction was warmed to room temperature, stirred for 2-4 hours and quenched with water. After removing THF, the residue was separated by ether or EtOAc and water. The separated organic layer was washed with brine, dried over sodium sulfate and concentrated to afford crude TMS-protected propargyl alcohol. Subsequently, a mixture of crude propargyl alcohol (1.0 equiv) and K ₂ CO ₃ (2.0 equiv) in methanol was stirred at room temperature for 0.5 to 1 hour. The solid was filtered off and washed with ether. The filtrate was concentrated, dissolved in ether, washed with water and brine and dried over sodium sulfate. Solvent was removed to give crude terminal acetylene product which was purified by distillation or chromatography (ethyl acetate / hexane). The total yield in this procedure is 62 to 97%.
[227] Examples of terminal alkyne prepared by the method are as follows:
[228] 3-ethynyl-3-hydroxy-piperidine-1-carboxylic acid tert-butyl ester,
[229] 4-ethynyl-4-hydroxy-piperidine-1-carboxylic acid tert-butyl ester,
[230] 3-ethynyl-3-hydroxy-pyrrolidine-1-carboxylic acid tert-butyl ester,
[231] Endo-α-3-ethynyl-3-hydroxy-8-aza-bicyclo [3.2.1] octane-8-carboxylic acid tert-butyl ester,
[232] Exo-α-3-ethynyl-3-hydroxy-8-aza-bicyclo [3.2.1] octane-8-carboxylic acid tert-butyl ester,
[233] 2- (1-hydroxy-prop-2-ynyl) -pyrrolidine-1-carboxylic acid tert-butyl ester,
[234] 1-cyclobutyl-prop-2-yn-1-ol,
[235] Pent-1-yn-3-ol,
[236] 4-amino-pent-1-yn-3-ol,
[237] 1- (3-aza-bicyclo [3.1.0] hex-6-yl) -prop-2-yn-1-ol,
[238] 4-ethynyl-tetrahydro-pyran-4-ol,
[239] (4-ethynyl-tetrahydro-pyran-4-yl) -carbamic acid tert-butyl ester,
[240] 2- (1-hydroxy-prop-2-ynyl) -piperidine-1-carboxylic acid tert-butyl ester,
[241] 3- (1-hydroxy-prop-2-ynyl) -piperidine-1-carboxylic acid tert-butyl ester,
[242] 4-ethynyl-1-methyl-piperidin-4-ol,
[243] (2-hydroxy-but-3-ynyl) -methyl-carbamic acid tert-butyl ester,
[244] (2-ethynyl-2-hydroxy-cyclohexyl) carbamic acid tert-butyl ester, and
[245] R- and S-3-ethynyl-1-aza-bicyclo [2.2.2] octan-3-ol.
[246] General Procedure for Homologization of Aldehydes to Terminal Alkynes
[247] To a cold (-78 ° C.) stirred LDA (lithium diisopropylamide) solution (1.3 equiv) in dry THF was added dropwise (1.3 equiv) a solution of (trimethylsilyl) diazomethane in hexane under nitrogen (free NH). For BOC-protected amino aldehydes, the amount of (trimethylsilyl) diazomethane and LDA is double). After 1 hour, aldehyde (1.0 equiv) was introduced into anhydrous THF and the cold bath was removed. The reaction was stirred at rt for 1-2 h, quenched with water, concentrated and separated into ether and water. The separated organic layer was washed with brine, dried over sodium sulfate and concentrated to afford the crude product, which was purified by chromatography (ethyl acetate / hexanes). The total yield in this procedure is 37 to 72%.
[248] Examples of terminal alkyne prepared by the method are as follows:
[249] 4-ethynyl-piperidine-1-carboxylic acid tert-butyl ester,
[250] 3 (S) -ethynyl-piperidine-1-carboxylic acid tert-butyl ester,
[251] 3 (R) -ethynyl-piperidine-1-carboxylic acid tert-butyl ester,
[252] 2-ethynyl-piperidine-1-carboxylic acid tert-butyl ester,
[253] 3-ethynyl-piperidine-1-carboxylic acid tert-butyl ester,
[254] 3-ethynyl-azetidine-1-carboxylic acid tert-butyl ester,
[255] (4-ethynyl-tetrahydro-pyran-4-yl) -carbamic acid tert-butyl ester, and
[256] [1- (tert-butyl-dimethyl-silanyloxymethyl) -prop-2-ynyl] -carbamic acid tert-butyl ester.
[257] 4-prop-2-ynyl-piperazin-1-carboxylic acid tert-butyl ester
[258] Potassium carbonate (3.70 g, 26.8 mmol) was added to a solution of N-t-butoxycarbonylpiperadine (5.0 g, 26.8 mmol) in acetone (40 mL). To the reaction mixture was added propargyl bromide (2.39 mL, 26.8 mmol) in acetone (10 mL) dropwise. The resulting mixture was stirred overnight at room temperature. Water is added, the aqueous layer is extracted with ether, the combined organic layers are washed with brine, dried over sodium sulfate and concentrated in vacuo to give 4-prop-2-ynyl-piperazine-1-carboxylic acid tert-butyl ester Was obtained, which was subjected to a Pd coupling reaction with an anilinoquinazoline suitable as crude material.
[259] Examples of terminal alkyne prepared by the method are as follows:
[260] 1-prop-2-ynyl-pyrrolidine,
[261] 3-methyl-4-prop-2-ynyl-piperazine-1-carboxylic acid tert-butyl ester,
[262] 3,5-dimethyl-4-prop-2-ynyl-piperazine-1-carboxylic acid tert-butyl ester,
[263] 1-methyl-4-prop-2-ynyl-piperazine,
[264] 4-prop-2-ynyl-morpholine,
[265] (3-prop-2-ynyl-3-aza-bicyclo [3.1.0] hex-6-yl) -methanol,
[266] 1-prop-2-ynyl-piperidin-4-ol,
[267] 1-prop-2-ynyl-piperidin-3-ol,
[268] 1-prop-2-ynyl-pyrrolidin-3-ol,
[269] (1-prop-2-ynyl-piperidin-4-yl) -methanol,
[270] (1-prop-2-ynyl-piperidin-3-yl) -methanol,
[271] (1-prop-2-ynyl-piperidin-2-yl) -methanol,
[272] (1-prop-2-ynyl-pyrrolidin-2-yl) -methanol,
[273] 2- (1-prop-2-ynyl-piperidin-4-yl) -ethanol,
[274] 2- (4-prop-2-ynyl-piperazin-1-yl) -ethanol,
[275] 4,4-dimethoxy-1-prop-2-ynyl-piperidine,
[276] 1-prop-2-ynyl-piperidin-4-ylamine,
[277] 2- (methyl-prop-2-ynyl-amino) -ethanol,
[278] 4-prop-2-ynyl-piperazine-1-carboxylic acid methylamide,
[279] 1- (4-prop-2-ynyl-piperazin-1-yl) -ethanone,
[280] 4-prop-2-ynyl-piperazine-1-carboxamide, and
[281] 1-methanesulfonyl-4-prop-2-ynyl-piperazine.
[282] 2-Chloro-N-prop-2-ynyl-acetamide
[283] Propargyl amine (250 mg, 0.34 mL, 4.6 mmol) was dissolved in dichloromethane (10 mL) and cooled to 0 ° C. Chloro-acetyl chloride (256 mg, 0.18 mL, 2.3 mmol) was added dropwise to the solution, the solution was stirred for 30 minutes and allowed to warm to room temperature. The solution was washed twice with H ₂ O, dried over Na ₂ SO ₄ and the solvent was removed. 2-Chloro-N-prop-2-ynyl-acetamide (385 mg) was obtained as white crystals. ¹ H NMR (400 MHz; CDCl ₃ ) δ 2.27 (1 H, m), 4.07 (2 H, s), 4.09 (2 H, q, J = 2.5 Hz), 6.78 (1 H, br s).
[284] Examples of terminal acetylenes prepared by this method are as follows:
[285] N-prop-2-ynyl-acetamide,
[286] N-prop-2-ynyl-propionamide,
[287] Cyclopropanecarboxylic acid prop-2-ynylamide,
[288] 2,2-dimethyl-N-prop-2-ynyl-propionamide,
[289] N-prop-2-ynyl-methanesulfonamide,
[290] N-methyl-N-prop-2-ynyl-acetamide,
[291] N- (1-methyl-prop-2-ynyl) -acetamide,
[292] N- (1,1-dimethyl-prop-2-ynyl) -acetamide, and
[293] 2-methoxy-N-prop-2-ynyl-acetamide.
[294] 2- (tert-butoxycarbonylamino) -2-methyl-1-propanol
[295] 2-amino-2-methyl-1-propanol (8.9 g, 0.1 mol), di-tert-butyldicarbonate (22.0 g, 0.1 mol) and Na ₂ CO _{3 in} water / THF (150/150 mL) 21.0 g, 0.2 mol) was refluxed for 1 hour. After removal of THF, the residue was separated by ether (200 mL) and water (150 mL). The separated organic layer was washed with brine (100 mL), dried over sodium sulfate and concentrated to give 17.97 g (95) of 2- (tert-butoxycarbonylamino) -2-methyl-1-propanol as a waxy white solid. %) Was obtained. ¹ H NMR (CDCl ₃ ) δ 1.23 (s, 6 H), 1.41 (s, 9 H), 3.56 (s, 2 H).
[296] 2- (tert-butoxycarbonylamino) -2-methyl-1-propionaldehyde
[297] A 2- (tert-butoxycarbonylamino) -2-methyl-1-propanol solution (5.7 g, 30.0 mmol) in triethylamine (42 mL) was dried over anhydrous DMSO (dimethylsulfoxide) (50 mL) at room temperature. Sulfur trioxide pyridine complex mixture (14.3 g, 90.0 mmol) was added. The reaction mixture was stirred for 1 h under nitrogen and concentrated. The residue was dissolved in EtOAc (200 mL), washed with water (100 mL) and brine (100 mL), dried over sodium sulphate and concentrated to yield crude 2- (tert-butoxycarbonylamino as a yellow oil. ) -2-methyl propionaldehyde was obtained. This was distilled and purified to obtain 4.90 g (87%) of a waxy white solid. ¹ H NMR (CDCl ₃ ) δ 1.30 (s, 6 H), 1.41 (s, 9 H), 4.97 (br, 1 H), 9.40 (s, 1 H).
[298] 4,4-dimethyl-5-trimethylsilylethynyl-2-oxazolidinone
[299] A cold (-78 ° C.) stirred (trimethylsilyl) acetylene solution (4.42 g, 45.0 mmol) in anhydrous THF (20 mL) was treated with nBuLi (18 mL, 45.0 mmol) under nitrogen. The colorless solution was stirred for 30 minutes and then 2- (tert-butoxycarbonylamino) -2-methyl propionaldehyde (2.80 g, 15 mmol) in dry THF. The reaction was warmed to rt, stirred for 2 h and quenched with water. After removal of THF, the residue was separated by ether (150 mL) and water (100 mL). The separated organic layer was washed with brine (100 mL), dried over sodium sulfate and concentrated to give crude 4,4-dimethyl-5-trimethylsilylethynyl-2-oxazolidinone (100%) as a yellow oil. It was used in the next step.
[300] 4,4-dimethyl-5-ethynyl-2-oxazolidinone
[301] A mixture of 4,4-dimethyl-5-trimethylsilylethynyl-2-oxazolidinone (15.0 mL) and K ₂ CO ₃ (4.1 g, 30.0 mmol) in methanol (30.0 mL) was stirred for 30 minutes. The solid was filtered off and washed with ether. The filtrate was concentrated, dissolved in ether (100 mL), washed with water (50 mL) and brine (50 mL) and dried over sodium sulfate. The solvent was removed to give 1.10 g (53%) of 4,4-dimethyl-5-ethynyl-2-oxazolidinone as a yellow oil. ¹ H NMR (CDCl ₃ ) δ 1.37 (s, 3 H), 1.39 (s, 3 H), 2.68 (s, 1 H), 4.82 (s, 1 H), 6.00 (br s, 1 H).
[302] Preparation of 4-ethynyl-4-hydroxy-tetrahydro-pyran-2-carboxylic acid amide
[303] 4-oxo-3,4-dihydro-2H-pyran-2-carboxylic acid ethyl ester:
[304] ZnCl (0.63 g, 4.6 mmol) was dissolved in anhydrous THF (15 mL) and 1-methoxy-3- (trimethylsilyloxy) -1,3-butadiene (7.94 g, 46.0 in toluene (30 mL) at room temperature Mmol) and ethyl glyoxalate (7.05 g, 69.0 mmol). After stirring for 30 minutes, water (30 mL) and TFA (trifluoroacetic acid) (2 mL) were added and the mixture was stirred vigorously for 20 minutes. After concentration, the residue was separated with EtOAc (200 mL) and water (100 mL). The separated organic layer was washed with brine, dried over sodium sulfate and concentrated to give 8.0 g (100%) of brown oil, which was used in the next step without further purification. ¹ H NMR (CDCl ₃ ) δ 1.30 (t, 3 H), 2.85 (d, 2 H), 4.26 (q, 2 H), 5.00 (t, 1 H), 5.48 (d, 1 H), 7.39 ( d, 1 H).
[305] 4-oxo-tetrahydro-pyran-2-carboxylic acid ethyl ester:
[306] Mix a mixture of 4-oxo-3,4-dihydro-2H-pyran-2-carboxylic acid ethyl ester (8.0 g, 46.0 mmol) and Pd / C (10%, 0.20 g) in EtOAc (70 mL) overnight. Shake in a Parr bottle with hydrogen at psi and filter through a pad of celite. The filtrate was concentrated and the residue was distilled to give 2.62 g (33%) of a yellowish oil. ¹ H NMR (CDCl ₃ ) δ 1.29 (t, 3 H), 2.40 (d, 1 H), 2.58-2.75 (m 3 H), 3.79 (tt, 1 H), 4.23 (q, 2 H), 4.28 (m, 1H), 4.40 (m, 1H).
[307] 4-Hydroxy-4-trimethylsilanylethynyl-tetrahydro-pyran-2-carboxylic acid ethyl ester:
[308] A cold (-78 ° C.) stirred (trimethylsilyl) acetylene solution (1.80 g, 18.24 mmol) in anhydrous THF (30 mL) was treated with nBuLi (7.3 mL in hexane, 18.24 mmol) under nitrogen. The colorless solution was stirred for 30 minutes, then 4-oxo-tetrahydro-pyran-2-carboxylic acid ethyl ester (2.62 g, 15.2 mmol) was added to dry THF (30 mL). The reaction was warmed to rt, stirred for 2 h and quenched with water (30 mL). After THF was removed, the product was extracted with EtOAc (2 times, 60 mL). The combined organic layers were washed with brine, dried over sodium sulfate and concentrated to give 2.50 g (61%) of yellow oil. ¹ H NMR (CDCl ₃ ) δ 0.17 (s, 9 H), 1.30 (t, 3 H), 1.76-1.90 (m, 3 H), 2.25 (m, 1 H), 3.66 (tt, 1 H), 4.11-4.21 (m, 2H), 4.24 (q, 2H).
[309] 4-Ethynyl-4-hydroxy-tetrahydro-pyran-2-carboxylic acid amide:
[310] 4-Hydroxy-4-trimethylsilanylethynyl-tetrahydro-pyran-2-carboxylic acid ethyl ester (2.50 g, 9.25 mmol) was dissolved in MeOH (20 mL) in a pressure reaction tube and NH ₃ gas was added to the solution. It was passed for 10 minutes with stirring. The tube was capped tightly and the reaction stirred for 3 days. After the solvent was removed, 1.53 g (97%) of a yellow oil were obtained. ¹ H NMR (CD ₃ OD) δ 1.48 (t, 1 H), 1.70 (td, 1 H), 1.85 (d, 1 H), 2.30 (d, 1 H), 3.04 (s, 1 H), 3.29 (s, 1 H), 3.71 (t, 1 H), 3.98 (d, 1 H), 4.06 (dt, 1 H).
[311] Preparation Example of 2- (tert-butyl-dimethyl-silanyloxymethyl) -4-ethynyl-tetrahydro-pyran-4-ol
[312] 2-hydroxymethyl-tetrahydro-pyran-4-ol:
[313] To a cold (0 ° C.) stirred LiAlH ₄ suspension (3.42 g, 90.0 mmol) in dry THF (50 mL) was added dropwise ₄ -oxo-tetrahydro-pyran-2-carboxylic acid ethyl ester solution (5.17 g, 30.0 mmol). . After stirring for 1 hour, the reaction was quenched by the slow addition of water (3.4 mL), 15% NaOH (3.4 mL) and water (10.0 mL) in order. The inorganic salts were filtered off and extracted repeatedly with EtOAc while the product was absorbed on solids. The solvent was removed to give 2.42 g (61%) of a yellow oil. The crude mixture was used in the next step without purification.
[314] 2- (tert-butyl-dimethyl-silanyloxymethyl) -tetrahydro-pyran-4-ol:
[315] 2-hydroxymethyl-tetrahydro-pyran-4-ol (2.42 g, 18.3 mmol) in anhydrous CH ₂ Cl ₂ (50 mL), DMAP (4-dimethylaminopyridine) (90 mg, 0.74 mmol) and Et ₃ To a solution of N (2.04 g, 20.1 mmol) was added tert-butyldimethylsilyl chloride (2.76 g, 18.3 mmol) at room temperature. After stirring overnight, the reaction solution was quenched with brine (30 mL) and the separated aqueous layer was extracted with CH ₂ Cl ₂ (40 mL). The combined organic extracts were dried over sodium sulfate and concentrated. Purification by silica gel column using 30% EtOAc in hexanes gave 2.27 g (50%) of a colorless oil. ¹ H NMR (CDCl ₃ ) δ 0.04 (s, 6 H), 0.88 (s, 9 H), 1.21 (m, 1 H), 1.43 (m, 1 H), 1.82 (dt, 1 H), 2.00 ( dt, 1 H), 3.35 (m, 1 H), 3.51 (q, 1 H), 3.66 (q, 1 H), 3.79 (m, 1 H), 4.01 (m, 1 H).
[316] 2- (tert-butyl-dimethyl-silanyloxymethyl) -tetrahydro-pyran-4-one:
[317] A 2- (tert-butyl-dimethyl-silanyloxymethyl) -tetrahydro-pyran-4-ol solution (2.27 g, 9.21 mmol) in anhydrous DMSO / Et ₃ N (15/13 mL) was dried at room temperature. Treated with oxide pyridine complex (7.33 g, 46.1 mmol). After stirring for 1 hour, the reaction was concentrated and the residue was separated between EtOAc (100 mL) and water (50 mL). The separated organic layer was washed with brine (70 mL), dried over sodium sulfate and concentrated. Purification by silica gel column using 10-20% EtOAc in hexanes yielded 1.48 g (66%) of a colorless oil. ¹ H NMR (CDCl ₃ ) δ 0.05 (s, 6 H), 0.88 (s, 9 H), 2.32 (dt, 1 H), 2.41 (m, 2 H), 2.58 (m, 1 H), 3.62 ( m, 2H), 3.70 (d, 2H), 4.31 (m, 1H).
[318] 2- (tert-butyl-dimethyl-silanyloxymethyl) -4-trimethylsilanylethynyl-tetrahydro-pyran-4-ol:
[319] A cold (-78 ° C.) stirred (trimethylsilyl) acetylene solution (1.01 g, 10.3 mmol) in anhydrous THF (25 mL) was treated with nBuLi (4.12 mL in hexane, 10.3 mmol) under nitrogen. The colorless solution was stirred for 30 minutes, then 2- (tert-butyl-dimethyl-silanyloxymethyl) -tetrahydro-pyran-4-one (1.48 g, 6.06 mmol) was added to dry THF (25 mL). It was. The reaction was warmed to rt, stirred for 2 h and quenched with water (30 mL). After removing THF, the product was extracted with EtOAc (2 times, 50 mL). The combined organic layers were washed with brine, dried over sodium sulfate and concentrated to give 1.75 g (84%) of yellow oil. ¹ H NMR (CDCl ₃ ) δ 0.05 (s, 6 H), 0.16 (s, 9 H), 0.89 (s, 9 H), 1.43 (m, 1 H), 1.78 (td, 1 H), 1.83 ( d, 1 H), 1.94 (d, 1 H), 3.52-3.70 (m, 4 H), 4.00 (m, 1 H).
[320] 2- (tert-butyl-dimethyl-silanyloxymethyl) -4-ethynyl-tetrahydro-pyran-4-ol:
[321] 2- (tert-butyl-dimethyl-silanyloxymethyl) -4-trimethylsilanylethynyl-tetrahydro-pyran-4-ol (1.75 g, 5.1 mmol) and K ₂ CO ₃ (1.4 g, 10.2 mmol ) Was stirred for 30 minutes at room temperature. After concentration, the residue was separated with EtOAc (50 mL) and water (30 mL) and the separated aqueous layer was extracted with EtOAc. The combined organic layers were dried over sodium sulfate and concentrated to give 1.33 g (96%) of a light yellow oil. ¹ H NMR (CDCl ₃ ) δ 0.05 (s, 6 H), 0.88 (s, 9 H), 1.50 (m, 1 H), 1.78 (m, 1 H), 1.84 (d, 1 H), 2.01 ( m, 1H), 2.55 (s, 1H), 3.55-3.70 (m, 4H), 4.00 (m, 1H).
[322] 6-iodo-4-quinazolinone:
[323] A solution of 2-amino-5-iodobenzoic acid (26.3 g, 100 mmol) and formamidine acetate (13.5 g, 130 mmol) in ethanol (400 mL) was refluxed for 20 hours. After cooling to 0 ° C., the solid product was collected by filtration. Drying in vacuo gave 6-iodo-4-quinazolinone (22.0 g, 81%) as a gray crystalline solid. ¹ H NMR (400 MHz; DMSO-d ₆ ) δ 12.38 (br, s, 1 H), 8.35 (d, 1 H), 8.05-8.10 (m, 2 H), 7.43 (dd, 1 H). LRMS: 272.9 (MH < + >).
[324] 6-iodo-4-chloroquinazoline (12):
[325] To a stirred DMF solution (6.3 mL) in DCE (20 mL) cooled to 0 ° C. was added dropwise an oxalyl chloride solution (60 mL of a 2 M solution in DCE). After complete addition, the cold bath was removed and 6-iodo-3H-quinazolinone (10 g, 36.8 mmol) was added as a solid. The resulting mixture was heated to reflux under nitrogen for 3 hours. The reaction was carefully quenched with H ₂ O while cooling to room temperature. CH ₂ CH ₂ was added and the two layers were transferred to a separatory funnel. The aqueous layer was extracted with CH ₂ CH ₂ (twice, 50 mL) and the combined organic layers were dried (Na ₂ SO ₄ ). The solvent was removed in vacuo to yield a yellow solid which was triturated with diethyl ether to remove any residual impurities. The yellow solid produced by filtration was found to be pure by NMR. ¹ H NMR (CDCl ₃ , 400 MHz) δ 9.05 (s, 1 H), 8.65 (d, 1 H), 8.21 (dd, 1 H), 7.78 (d, 1 H).
[326] 6-iodo-4-phenoxyquinazolin (13):
[327] The NaH suspension in DMF (40 mL) (washed without mineral oil) was cooled to 0 ° C. and a phenol solution (5.65 g, 60 mmol) in DMF (20 mL) was added dropwise. After complete addition, 6-iodo-4-chloroquinazolin (14.6 g, 50.3 mmol) was added in small portions as a solid. The cold bath was transferred and the reaction mixture was stirred for 2 hours at room temperature. The mixture was then quenched with water (200 mL), diluted with EtOAc (300 mL) and transferred to a separatory funnel. The organic layer was washed with aqueous NaOH, water and brine and dried over Na ₂ SO ₄ . The solid was filtered and the solvent removed to give quinazoline (13) as a yellow solid (17.2 g, 98%). ¹ H NMR (400 MHz, CDCl ₃ ) δ 8.74 (d, 1 H), 8.14 (s, 1 H), 8.12 (dd, 1 H), 7.71 (d, 1 H), 7.49 (dd, 2 H) , 7.32 (t, 1H), 7.22 (m, 2H).
[328] Method A: (1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-imidazol-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine ( 15)
[329] (1-benzenesulfonyl-1H-indol-5-yl)-[6-iodo-quinazolin-4-yl] -amine (14):
[330] 6-iodo-4-chloroquinazolin (2.38 g, 8.20 mmol) and 5-amino-1-benzenesulfonylindole (2.46 g, 9.00 mmol) in DCE (20 mL) and t-butanol (20 mL) Mixed. The resulting mixture was heated at reflux under nitrogen for 18 hours to form a light yellow suspension. While cooling, the solid was filtered off, rinsed with CH ₂ CH ₂ and placed under high vacuum to remove any excess solvent. Quinazolin 14 (3.23 g, 75%) was obtained as a yellow solid. ¹ H NMR (DMSO d ₆ , 400 MHz) δ 9.24 (s, 1 H, NH), 8.84 (s, 1 H), 8.33 (dd, 1 H, 8.9 Hz, 1.7 Hz), 8.01 (m, 4 H ), 7.90 (m, 2H), 7.70 (m, 2H), 7.60 (m, 3H), 6.92 (dd, 1H, J = 3.7 Hz, 0.6 Hz).
[331] (1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-imidazol-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine (15):
[332] Quinazoline (14) (150 mg, 0.28 mmol), 1-N-2-propynimidazole (200 mg, 1.89 mmol), Pd (OAc) ₂ (4 mg, 0.016 mmol) and PPh ₃ (9 mg , 0.033 mmol) was mixed in NEt ₃ (1.25 mL) and DMF (0.5 mL). The mixture was heated at 80 ° C. under N ₂ for 16 h. While cooling, the black suspension was concentrated under reduced pressure and the residue was dissolved in MeOH. Silica gel (1 g) was added and methanol was removed in vacuo. The resulting silica gel was placed on top of a silica gel (40 g) column, eluted with 200 mL of 50: 1 CH ₂ CH ₂ : MeOH and 300 mL of 25: 1 CH ₂ CH ₂ : MeOH to give alkyne as a yellow foam. (15) was obtained (72 mg, 51%). ¹ H NMR (CDCl ₃ , 400 MHz) δ 8.95 (br, 1 H, NH), 8.63 (s, 1 H), 8.62 (s, 1 H), 8.24 (s, 1 H), 7.96 (d, 1 H, J = 1.7 Hz), 7.84 (m, 3 H), 7.71 (m, 2 H), 7.51 (m, 3 H), 7.41 (m, 2 H), 7.14 (s, 1 H), 7.10 ( s, 1 H), 6.55 (d, 1 H, J = 3.5 Hz), 5.01 (s, 2 H).
[333] Method A ′: (3-Methyl-4-phenoxy-phenyl)-[6- (3-piperazin-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine
[334] (6-iodo-quinazolin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine:
[335] 4-chloro-6-iodo-quinazolin (5.0 g, 17.2 mmol) and 3-methyl-4-phenoxyaniline (17.2 mmol) were mixed with 1: 1 dichloroethane and t-butanol (50 mL) It was. The reaction mixture was heated at 90 ° C. for 4 hours, whereby a yellow precipitate was observed. The reaction was cooled and the precipitate collected and (6-iodo-quinazolin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine was obtained (8.0 g, 94%). M / z, 454. ¹ H NMR (CD ₃ OD) δ 9.12 (s, 1 H), 8.83 (s, 1 H), 8.39 (d, 1 H, J = 8.8 Hz), 7.63 (d, 1 H , J = 8.8 Hz), 7.55 (d, 1 H, J = 2.1 Hz), 7.35 (dd, 1 H, J1 = J2 = 8.5 Hz), 7.28 (t, 2 H, J = 8.1 Hz), 7.05 ( t, J = 8.5 Hz), 6.87 (d, 1H, J = 8.1 Hz), 3.81 (s, 3H).
[336] (3-Methyl-4-phenoxy-phenyl)-[6- (3-piperazin-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine:
[337] 4-prop-2-ynyl-piperazine-1-carboxylic acid tert-butyl ester (2.37 g, crude compound) and (6-iodo-quina in Et ₃ N (8 mL) and DMF (3 mL) Zolin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine (800 mg, 1.76 mmol), Pd (OAc) ₂ (23.7 mg, 0.105 mmol) and PPh ₃ (55.3 mg, 0.21 mmol) ) Were mixed together. The resulting reaction mixture was heated at 80 ° C overnight. After cooling, methylene chloride was added to the reaction mixture, and the dark mixture was washed with brine and dried over sodium sulfate. The solvent was removed and the residue was chromatographed on silica gel (1: 1 hexanes and ethyl acetate) to afford product (2). When product (2) was dissolved in methylene chloride, HCl gas was generated for 5 minutes, and the precipitate was collected by (3-methyl-4-phenoxy-phenyl)-[6- (3-piperazin-1-yl- Prop-1-ynyl) -quinazolin-4-yl] -amine was obtained (400 mg, 46.7%).
[338] ^{M / z, 450. 1 H NMR} (DMSO) δ (ppm) 9.52 (s, 1 H), 8.84 (s, 1 H), 8.20 (dd, 1 H, J1 = 8.7 Hz, J2 = 1.3 Hz), 7.99 (d, 1 H, J = 2.5 Hz), 7.60 (dd, 1 H, J = 8.7 Hz, J2 = 2.7 Hz), 7.36 (about t, 2 H, J = 8.5 Hz), 7.11 (t, 1 H, J = 7.5 Hz), 6.92 (d, 1 H, J = 8.8 Hz), 6.91 (d, 1 H, J = 7.9 Hz), 3.55 (br, 4 H), 3.44 (br, 4 H), 3.30 (s, 2H), 2.19 (s, 3H).
[339] Method B: (6-cyclobutyl-quinazolin-4-yl)-(4-phenoxy-phenyl) amine (17)
[340] (6-Cyclobutyl-4-phenoxyquinazolin (16):
[341] Agitated naphthalene (3.85 g, 30 mmol) in anhydrous THF (tetrahydrofuran) (20 mL) was added a small amount of finely divided lithium metal (0.21 g, 30 mmol) at room temperature. The mixture turned dark green and stirring continued for 2 hours. A ZnCl ₂ solution (33 mL of 0.5 M solution in THF, 16.5 mmol) was added dropwise with a syringe to give a black color. After 3 hours, stirring was stopped, resulting in the precipitation of fine Zn dust. The supernatant (about 40 mL) was removed with a dry pipette and replaced with fresh THF (10 mL). Cyclobutyl bromide (2.0 g, 14.8 mmol) was added and the resulting dark mixture was stirred at rt for 16 h. Agitation was stopped again and the supernatant organozinc reagent was used immediately for the next reaction.
[342] 6-iodo-4-phenoxyquinazolin (1.75 g, 5.03 mmol) in THF (10 mL), Pd ₂ (dba) ₃ [tris (dibenzylideneacetamide) dipalladium (0)] (90 mg, 0.1 mmol) and tripurylphosphine (185 mg, 0.8 mmol) were added cyclobutyl zinc as above. The resulting mixture was stirred for 6 h, then diluted with THF (30 mL) and quenched with saturated NH ₄ Cl solution (40 mL). The two layers were separated and the organic layer was washed with water and brine and dried (Na ₂ SO ₄ ). The solids were removed and the solvent removed in vacuo to yield a brown oil. Purification by silica gel chromatography eluting with 1: 1 EtOAc: hexanes gave 6-cyclobutyl-4-phenoxyquinazolin as a yellow oil (0.78 g, 56%). ¹ H NMR (400 MHz: CDCl ₃ ) δ 8.71 (s, 1 H), 8.14 (s, 1 H), 7.92 (d, 1 H), 7.78 (dd, 1 H), 7.50 (t, 2 H) , 7.31 (t, 1 H), 7.25 (d, 2 H), 3.78 (m, 1 H), 2.43 (m, 2 H), 2.25 (m, 2 H), 2.11 (m, 1 H), 1.92 (m, 1 H).
[343] (6-Cyclobutyl-quinazolin-4-yl)-(4-phenoxy-phenyl) amine (17):
[344] Quinazolin (16) (50 mg, 0.18 mmol) was mixed with 4-phenoxyaniline (67 mg, 0.36 mmol) in phenol (0.45 g). The mixture was heated at 100 ° C. for a total of 17 hours. Excess phenol was distilled off under reduced pressure to give a residue, which was triturated with CH ₂ Cl ₂ to give the desired quinazoline (17) as a yellow solid (20 mg, 30%). ¹ H NMR (DMSO d ₆ , 400 MHz) δ 9.76 (s, 1 H), 8.47 (s, 1 H), 8.31 (s, 1 H), 7.77 (d, 2 H), 7.69 (m, 2 H ), 7.36 (t, 2H), 7.11 (t, 1H), 7.03 (d, 2H), 6.98 (d, 2H), 3.69 (m, 1H), 2.35 (m, 2H), 2.23 (m, 2H), 2.01 (m, 1H), 1.86 (m, 1H).
[345] Method C: cis- and trans-3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -cyclobutanecarboxylic acid ethyl ester (19a / 19b)
[346] Cis- and trans-3- (4-phenoxy-quinazolin-6-yl) -cyclobutanecarboxylic acid ethyl ester (18a, b):
[347] To the naphthalene solution (1.92 g, 15 mmol) in anhydrous THF was added a small amount of finely cut Li metal (104 mg, 15 mmol) under N ₂ to give a green mixture which was stirred for 2 hours. Zinc chloride (16 mL of 0.5 M solution in THF, 8 mmol) was added dropwise by syringe and the mixture was stirred at room temperature for 3 hours. Agitation was stopped, the supernatant was removed and replaced with ethyl-3-iodocyclobutane-1-carboxylate solution (790 mg, 3 mmol). The resulting supernatant was stirred for 20 hours when stirring was stopped, resulting in precipitation of residual Zn metal. The remaining solution was placed in a dry flask containing quinazoline (13) (520 mg, 1.5 mmol), Pd ₂ (dba) ₃ (27 mg, 0.03 mmol) and tri-2-furylphosphine (56 mg, 0.24 mmol). Moved. The mixture was stirred at rt for 16 h. The mixture was concentrated and the residue was taken up in EtOAc (30 mL), washed with saturated aqueous NH ₄ Cl, brine and H ₂ O and dried (Na ₂ SO ₄ ). The solvent was removed in vacuo and the resulting residue was purified by silica gel chromatography to give cyclobutyl esters (18a, 18b) as a mixture of cis and trans isomers (300 mg, 57%). LRMS: 349.2 (MH < + >). HPLC: 7.31 min (28%); 7.44 min (72%).
[348] Cis- and trans-3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -cyclobutanecarboxylic acid ethyl ester (19a, b):
[349] Ester (300 mg, 0.86 mmol) was mixed with 5-amino-1-phenylsulfonylindole (270 mg, 1.0 mmol) and phenol (1.0 g). The mixture was heated to 100 ° C for 48 h. Excess phenol was distilled off and the residue was dissolved in CH ₂ Cl ₂ , transferred to a separating funnel and washed with H ₂ O and brine. The organic layer was dried (Na ₂ SO ₄ ) and the solvent was removed to give a dark residue which was purified by preparative TLC eluting with EtOAc to give esters (19a, 19b) as waxy solids (0.20 g). , 44%). LRMS: 527.2 (MH < + >). HPLC: 7.54 min (16%); 7.64 min (84%).
[350] Method D: cis- and trans- [3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -cyclobutyl] -methanol (20a, b)
[351] DIBAL-H (diisobutylaluminum hydride) (1 M in toluene) via syringe in cold (-78 ° C.) stirred ethyl ester solution (19a / 19b) (70 mg, 0.13 mmol) in anhydrous toluene (5 mL). ) 0.78 ml was added. The reaction was then warmed up to 0 ° C., stirred for 3 h and then quenched by dilution with aqueous NH ₄ Cl. The mixture was transferred to a separatory funnel and extracted with ethyl acetate. The organic layer was dried (Na ₂ SO ₄ ), the solids were removed and the remaining filtrate was concentrated to give an oil which was purified by preparative TLC (eluted with w / ethyl acetate) to give alcohol (20a, 20b) 7 mg (11%) was obtained. MS m / z (MH <+>). 485.2; HPLC: 5.97 min.
[352] Method E: cis- and trans- [3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -cyclobutyl] -pyrrolidin-1-yl -Metanon (21a, b)
[353] Ethyl ester (19a / 19b) (60 mg, 0.11 mmol) was dissolved in methanol (5 mL) and refluxed for 1 hour to convert the ethyl ester to methyl ester. After the methanol was removed, the residual salt solution was dissolved in pyrrolidine (5 mL) and heated at reflux for 20 hours. Pyrrolidine was removed to give an oily brown product mixture, which was purified by preparative TLC (ethyl acetate eluting) to give 22 mg (36%) of amides (21a, 21b) as waxy yellow solids. MS m / z (MH <+>). 552.2; HPLC: 6.447 min.
[354] Method F: 4- [4- (1-benzyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol (23)
[355] 1-Methyl-4- (4-phenoxy-quinazolin-6-ylethynyl) -piperidin-4-ol (22):
[356] In a 100 ml round bottom flask, quinazoline (13) (1.32 g, 3.80 mmol), 4-ethynyl-1-methyl-piperidin-4-ol (1.06 g, 7.6 mmol), Pd (OAc) ₂ under nitrogen (51 mg, 0.23 mmol), PPh ₃ (120 mg, 0.46 mmol) and triethylamine (18 mL) were added. The flask was equipped with a reflux condenser and the mixture was heated to 100 ° C for 16 h. The dark solution was cooled down and triethylamine was removed under reduced pressure. The resulting residue was diluted with EtOAc (75 mL) and H ₂ O (25 mL) and transferred to a separatory funnel. The organic layer was washed twice with H ₂ O (25 mL) and the combined aqueous washes were back extracted with EtOAc (25 mL). The combined organic layers were dried (MgSO ₄ ) and the solvent removed under reduced pressure. The resulting black foam was yellow by purification over silica gel (50 g) eluting with 250 mL of 30: 1 CH ₂ Cl ₂ : MeOH and then 400 mL of 30: 1: 1 CH ₂ Cl ₂ : MeOH: NEt ₃ . Obtained the desired product as a foam (930 mg, 68%). ¹ H NMR (CDCl ₃ ; 400 MHz) δ 8.71 (s, 1 H), 8.36 (d, 1 H, J = 1.9 Hz), 7.89 (d, 1 H, J = 8.7 Hz), 7.80 (dd, 1 H, J = 8.7 Hz, 1.9 Hz), 7.45 (t, 2 H, J = 8.3 Hz), 7.31 (m, 1 H), 7.21 (m, 2 H), 2.72 (br, 2 H), 2.47 ( br, 2H), 2.31 (s, 3H), 2.09 (m, 2H), 2.00 (m, 2H).
[357] 4- [4- (1-Benzyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol (23):
[358] In 1 ml of a Wheaton vial, quinazoline (22) (80 mg, 0.22 mmol) was replaced with amino-1-benzylindole (54 mg, 0.24 mmol), pyridinium hydrochloride (5 mg, 0.04 mmol) and phenol (104 mg, 1.11 mmol). The vial was capped and heated at 100 ° C. for 16 hours. After cooling the contents of the Weaton vial, it was solvated in a small amount of EtOAc and placed on top of a silica gel (5 g) column. The column was eluted with hexanes: EtOAc / NEt ₃ in 1: 1: 0.1 to remove high R _f impurities. The desired product (23) (R _f 0.05, 10: 1 CH ₂ Cl ₂ : MeOH) was eluted with 10: 1 CH ₂ Cl ₂ : MeOH to give a yellow solid (65 mg, 60%). ¹ H NMR (DMSO d ₆ , 400 MHz) δ 9.88 (s, 1 H, NH), 8.67 (s, 1 H), 8.45 (s, 1 H), 7.92 (d, 1.7 Hz), 7.76 (d, 1 H, J = 8.5 Hz), 7.67 (d, 1 H, J = 8.5 Hz), 7.50 (d, 1 H, J = 3.1 Hz), 7.42 (d, 1 H, J = 8.9 Hz), 7.35 ( dd, 1 H, J = 8.9 Hz, 1.9 Hz), 7.31-7.18 (m, 6 H), 6.48 (dd, 1 H, J + 3.1 Hz, 0.8 Hz), 5.41 (s, 2 H), 2.97 ( br, 2H), 2.67 (br, 2H), 2.47 (s, 3H), 1.92 (br, 2H), 1.82 (br, 2H). LRMS: 488.2 (MH &lt; + &gt;), 126.1.
[359] Method G: Acetic acid 3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -allyl ester (27)
[360] 3- (4-Phenoxy-quinazolin-6-yl) -acrylic acid methyl ester (24):
[361] Quinazoline (13) (3.5 g, 10.0 mmol), methyl acrylate (6.0 g, 70.0 mmol), Pd (OAc) ₂ (140 mg, 0.62 mmol), PPh ₃ (320 mg, 1.22 mmol) in a pressure bottle, DMF (4 mL) and NEt ₃ (15 mL) were charged. The tube was purged with nitrogen, sealed and heated at 110 ° C. with stirring for 3 hours. The mixture was cooled, diluted with EtOAc, transferred to a separatory funnel, washed with H ₂ O and dried (MgSO ₄ ). After filtration, the filtrate was concentrated under reduced pressure to give a yellow solid which was recrystallized (EtOAc) to give ester 24 as a light yellow solid (2.2 g, 72%). ¹ H NMR (CDCl ₃ ; 400 MHz) δ 8.76 (s, 1 H), 8.47 (s, 1 H), 8.08 (d, 1 H), 8.06 (d, 1 H), 7.87 (dd, J = 16 Hz, 1 Hz), 7.48 (t, 2 H), 7.35 (t, 1 H), 7.25 (m, 2 H), 6.60 (d, J = 16 Hz, 1 Hz), 3.83 (s, 3 H) .
[362] 3- (4-phenoxy-quinazolin-6-yl) -prop-2-en-1-ol (25):
[363] Ester 24 (1.35 g, 4.41 mmol) in toluene (60 mL) was added dropwise DIBAL-H (8.8 mL, 8.8 mmol) of 1 M solution in toluene under N ₂ at −78 ° C. The reaction was then warmed to 0 ° C., stirred for 30 minutes, then quenched with 30 ml of saturated Rochelle's salt and the mixture was stirred overnight. The two layers were transferred to a separatory funnel and the organic layer was washed with H ₂ O and brine and dried (MgSO ₄ ). After filtration, the organic layer was concentrated under reduced pressure to give a yellow oil which was purified by silica gel chromatography eluting with 1: 1 hexanes: EtOAc and then EtOAc. Allyl alcohol (25) (900 mg, 73%) was isolated as a pale yellow oil. ¹ H NMR (CDCl ₃ ; 400 MHz) δ 8.72 (s, 1 H), 8.27 (s, 1 H), 7.66 (m, 2 H), 7.62 (m, 1 H), 7.47 (m, 3 H) , 7.34 (m, 1H), 7.24 (m, 2H), 6.82 (dd, 1H), 6.56 (m, 1H), 4.41 (dd, 1H).
[364] Acetic acid 3- (4-phenoxy-quinazolin-6-yl) -allyl ester (26):
[365] To alcohol (25) (900 mg, 3.23 mmol) and pyridine (0.8 mL, 10 mmol) in anhydrous CH ₂ Cl ₂ (15 mL) was added acetyl chloride (0.3 mL, 4.2 mmol) at 0 ° C. The resulting mixture was stirred for 2 hours and diluted with CH ₂ Cl ₂ (10 mL) and 5% HCl (10 mL). The mixture was transferred to a separatory funnel and the organic layer was washed with H ₂ O and brine. The organic layer was dried (Na ₂ SO ₄ ), the solids filtered off and the solvent removed in vacuo to yield the desired acetate as a yellow waxy solid. (26) was obtained (1.04 g, 100%). ¹ H NMR (CDCl ₃ ; 400 MHz) δ 8.72 (s, 1 H), 8.30 (d, 1 H, J = 1.7 Hz), 7.98 (m, 2 H), 7.49 (m, 2 H), 7.30 ( m, 1 H), 7.25 (m, 2 H), 6.84 (d, 1 H, J = 16.0 Hz), 6.46 (m, 1 H), 4.79 (dd, 2 H, J = 6.2 Hz, 1.2 Hz) , 2.11 (s, 3H).
[366] Acetic acid 3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -allyl ester (27):
[367] A mixture of ester 26 (630 mg, 1.97 mmol) and 5-amino-1-phenylsulfonylindole in phenol (3.0 g) was heated at 100 ° C. for 20 hours. Excess phenol was distilled off and the resulting brown oil was purified by silica gel chromatography eluting with 1: 1 ethyl acetate: hexanes and then ethyl acetate. Quinazolin (27) was obtained as an off-white waxy solid (430 mg, 43%). ¹ H NMR (CDCl ₃ ; 400 MHz) δ 8.61 (s, 1 H), 7.92 (m, 3 H), 7.82 (m, 4 H), 7.51 (m, 2 H), 7.43 (m, 3 H) , 6.74 (d, 1 H), 6.62 (d, 1 H), 6.45 (dt, 1 H), 4.74 (dd, 2 H), 2.09 (s, 3 H).
[368] Method G ': 3- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-yl] -acrylic acid methyl ester (28) and 3- [4- (4-phenoxy -Phenylamino) -quinazolin-6-yl] -prop-2-en-1-ol (29)
[369] 4-phenoxyquinazoline intermediates (24, 25) were converted to 4-arylaminoquinazoline derivatives (28, 29), respectively, using the same procedure used to transform intermediate (26) to (27).
[370] Method H: [6- [3- (6-Amino-3-aza-bicyclo [3.1.0] hex-3-yl) -propenyl] -quinazolin-4-yl]-(1-benzenesulfonyl -1H-indol-5-yl) -amine (30)
[371] A mixture of palladium acetate (6 mg, 0.027 mmol) and P (C ₆ H ₄ -m- SO ₃ Na) ₃ (30 mg, 0.053 mmol) in water (0.3 mL) was stirred at rt for 1 h, then CH Allylic acetate (16) (150 mg, 0.30 mmol) and (1a, 5a, 6a) -6-t-butyloxycarbonylamino-3-azabicyclo [3.1.0] hexane in ₃ CN (3 mL) [ Prepared according to Brighty et al., "Synlett 1996, pp. 1097-1099" (71 mg, 0.36 mmol). The resulting reaction mixture was stirred for 1.5 h at 50 ° C., dissolved in ethyl acetate (10 mL) and washed with aqueous NH ₄ Cl and water. The separated organic layer was dried over Na ₂ SO ₄ and concentrated to give a brown oil. Purification by preparative TLC (ethyl acetate eluting) gave 31 mg of a yellow solid. The BOC-protected product obtained was dissolved in methanol (5 mL) and deprotected by passing HCl gas through the solution with stirring. After concentration under high vacuum and drying, amine 30 was obtained as its HCl salt (18 mg, 11%). MS m / z (MH ⁺ ) 537.2; HPLC 4.423 min.
[372] Method I: 4- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol hydrochloride (32)
[373] 4- (4-Chloro-quinazolin-6-ylethynyl) -tetrahydro-pyran-4-ol (31):
[374] 4-ethynyl-4-hydroxytetrahydropyran (70 mg, 0.55 mmol), 4-chloro-6-iodoquinazoline (145 mg, 0.50 mmol), bis (triphenylforce) in anhydrous THF (5 mL) FIN) Purge a mixture of palladium (II) chloride (24 mg, 7 mol%), copper (I) iodide (6.6 mg, 7 mol%) and diisopropylamine (56 mg, 0.55 mmol) with N ₂ and stirred for 2 hours under a N ₂ atmosphere. After dilution with ethyl acetate (30 mL), the mixture was washed with NH ₄ Cl, H ₂ O and brine, dried over Na ₂ SO ₄ and concentrated to give the product as a yellow solid. Crystallization from ethyl acetate / hexanes yielded 0.13 g (90%) of a tan solid. ¹ H NMR (CD ₃ OD) δ 1.88 (m, 2 H), 2.04 (m, 2 H), 3.73 (m, 2 H), 3.91 (m, 2 H), 8.04 (s, 1 H), 8.05 (s, 1 H), 8.36 (s, 1 H), 9.00 (s, 1 H).
[375] 4- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl) -tetrahydro-pyran-4-ol hydrochloride (32)
[376] 4- (4-Chloro-quinazolin-6-ylethynyl) -tetrahydro-pyran-4-ol (43 mg, 0.15 mmol) in 2 ml of t-BuOH / 1,2-dichloroethane (1: 1) And a mixture of 4-phenoxyaniline (28 mg, 0.15 mmol) were heated at 90 ° C. with stirring in the reaction vial for 1 hour. The reaction was cooled, diluted with CH ₂ Cl ₂ and the product collected by filtration to give 52 mg of product 32 as a yellow solid. ¹ H NMR (CD ₃ OD) δ 1.86 (m, 2 H), 2.02 (m, 2 H), 3.74 (m, 2 H), 3.92 (m, 2 H), 7.05 (m, 4 H), 7.15 (t, J = 7.6 Hz, 1 H), 7.38 (t, J = 7.6 Hz, 2 H), 7.69 (d, J = 6.8 Hz, 2 H), 7.81 (d, J = 7.2 Hz, 1 H) , 8.07 (d, J = 7.2 Hz, 1H), 8.75 (s, 2H); HPLC: 6.36 min.
[377] Method J: (3-methoxy-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl) -amine
[378] 4- (4-Chloro-quinazolin-6-ylethynyl) -piperidine-1-carboxylic acid tert-butyl ester:
[379] 4-ethynyl-piperidine-1-carboxylic acid tert-butyl ester (1.12 g, 5.35 mmol), 4-chloro-6-iodoquinazoline (1.35 g, 4.65 mmol) in dry THF (20 mL), A mixture of dichlorobis (triphenylphosphine) palladium (II) (0.16 g, 0.23 mmol), copper (I) iodide (0.044 g, 0.23 mmol) and diisopropylamine (0.47 g, 4.65 mmol) was purged with nitrogen. Stirred at room temperature under 2 hours. After concentration, the residue was dissolved in CH ₂ Cl ₂ (100 mL), washed with aqueous NH ₄ Cl and brine, dried over sodium sulfate and concentrated to give the crude product as a brown oil. Purification by silica gel column using 20% EtOAc in hexanes yielded 1.63 g (94%) of tacky yellow oil. ¹ H NMR (CDCl ₃ ) δ 1.45 (s, 9 H), 1.67-1.75 (m, 2 H), 1.87-1.92 (m, 2 H), 2.84 (m, 1 H), 3.20-3.26 (m, 2 H), 3.78 (br d, 2 H), 7.88 (dd, 1 H), 7.97 (d, 1 H), 8.26 (d, 1 Hz), 9.00 (s, 1 H).
[380] (3-methoxy-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl) -amine:
[381] 4- (4-Chloro-quinazolin-6-ylethynyl) -piperidine-1-carboxylic acid tert-butyl ester (131 mg, 0.304) in t-BuOH / ClCH ₂ CH ₂ Cl (1.0 / 1.0 mL) Mmol) and a solution of 3-methoxy-4-phenoxyaniline hydrochloride (77 mg, 0.306 mmol) were heated for 30 minutes at 90 ° C. in a capped vial. After cooling, the yellow mixture was diluted with MeOH and HCl gas passed through the mixture for 10 minutes. After stirring for 2 hours, EtOAc was added to precipitate more solids which were collected by suction filtration, rinsed with EtOAc and further dried to give 105 mg (66%) of a yellow solid. ¹ H NMR (CD ₃ OD) δ 1.93-2.02 (m, 2H), 2.18-2.24 (m, 2H), 3.12-3.21 (m, 2H), 3.41-3.47 (m, 2H), 3.81 (s, 3H), 6.87 (d, 2H), 7.02 (t, 1H), 7.06 (d, 1H), 7.27 (t, 2H), 7.33 (dd, 1H), 7.56 (d , 1 H), 7.80 (d, 1 H), 8.06 (d, 1 H), 8.79 (s, 1 H), 8.83 (s, 1 H); MS m / z (MH ⁺ ) 451.3.
[382] Method K: (3-Methyl-4-phenoxy-phenyl)-[6- (1-propyl-piperidin-3-ylethynyl) -quinazolin-4-yl] -amine
[383] (3-Methyl-4-phenoxy-phenyl)-[6- (1-propyl-piperidin-3-ylethynyl) -quinazolin-4-yl] -amine:
[384] (3-Methyl-4-phenoxy-phenyl)-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine (114 mg, 0.2 mmol) and propionaldehyde (116 mg, 2.0 Mmol) was dissolved in MeOH / H ₂ O (5 / 0.5 mL) and the pH was adjusted to 5 using AcOH. The reaction was stirred overnight at room temperature and then NaBH ₃ CN (13 mg, 0.2 mmol) was added over 1 hour. After stirring for a few more hours, the reaction was concentrated and the residue was separated into CH ₂ Cl ₂ (30 mL) and saturated Na ₂ CO ₃ (20 mL). The separated organic layer was dried over sodium sulfate and concentrated. Purification by preparative TLC using 10% MeOH in EtOAc gave the free base product, which was converted to HCl salt to give 42 mg (38%) of a yellow solid. ¹ H NMR (CD ₃ OD) δ 1.03 (t, 3 H), 1.78-1.87 (m, 4 H), 2.01-2.08 (m, 2 H), 2.28 (s, 3 H), 2.96 (t, 1 H), 3.07-3.19 (m, 3H), 3.31 (br, 1H), 3.59 (d, 1H), 3.80 (d, 1H), 6.94 (m, 3H), 7.09 (t, 1 H), 7.34 (t, 2H), 7.53 (d, 1H), 7.63 (s, 1H), 7.80 (d, 1H), 8.05 (dd, 1H), 8.73 (s, 1H) , 8.75 (s, 1 H); MS m / z (MH ⁺ ) 477.1.
[385] Method K ': [6- [1- (2-Amino-ethyl) -piperidin-3-ylethynyl] -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl)- Amine
[386] [6- [1- (2-Amino-ethyl) -piperidin-3-ylethynyl] -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine:
[387] (3-Methyl-4-phenoxy-phenyl)-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine (114 mg, 0.2 mmol) and tert-butyl N- ( 2-oxoethyl) carbamate (320 mg, 2.0 mmol) was dissolved in MeOH / H ₂ O (5 / 0.5 mL) and the pH was adjusted to 5 using AcOH. The reaction was stirred overnight at room temperature, then NaBH ₃ CN (13 mg, 0.2 mmol) was added over 1 hour. After stirring for a few more hours, the reaction was concentrated and the residue was separated into CH ₂ Cl ₂ (30 mL) and saturated Na ₂ CO ₃ (20 mL). The separated organic layer was dried over sodium sulfate and concentrated. Purification by preparative TLC using 5% MeOH in EtOAc gave the free base, which was dissolved in MeOH. HCl gas was passed through the solution for 5 minutes and the deprotected product was precipitated as HCl salt. The mixture was diluted with EtOAc and the solid collected by suction filtration, rinsed with EtOAc and further dried to give 83 mg (71%) of a yellow solid. ¹ H NMR (CD ₃ OD) δ 1.71-1.82 (br, 2 H), 2.0-2.12 (br, 2 H), 2.27 (s, 3 H), 3.00 (t, 1 H), 3.03-3.19 (br , 2 H), 3.40 (br, 1 H), 3.50 (s, 2 H), 3.62 (br d, 1 H), 3.70 (m, 1 H), 3.89 (br d, 1 H), 6.93 (m , 3 H), 7.08 (t, 1 H), 7.33 (t, 2 H), 7.52 (d, 1 H), 7.64 (s, 1 H), 7.79 (d, 1 H), 8.05 (d, 1 H), 8.75 (s, 1 H), 8.77 (s, 1 H); MS m / z (MH ⁺ ) 476.1.
[388] Method L: 3- [2- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol
[389] 3- [2- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol:
[390] 3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol dihydrochloride (100 mg, 0.19 mmol) with Pd / A mixture of C (10%, 6 mg) was shaken in a wave bottle with hydrogen at 50 psi overnight and filtered through a pad of celite. The filtrate was concentrated to a small volume and added dropwise into EtOAc with stirring. The solid was collected by suction filtration, rinsed with EtOAc and further dried to give 89 mg (89%) of a yellow solid. ¹ H NMR (CD ₃ OD) δ 1.69 (dt, 1 H), 1.81 (br d, 1 H), 1.95 (t, 3 H), 2.15 (m, 1 H), 2.28 (t, 3 H), 2.93 (t, 1 H), 3.02 (m, 3 H), 3.18 (d, 1 H), 3.31 (br, 1 H), 6.94 (m, 3 H), 7.08 (t, 1 H), 7.34 ( t, 2 H), 7.55 (d, 1 H), 7.66 (d, 1 H), 7.78 (d, 1 H), 8.02 (d, 1 H), 8.58 (s, 1 H), 8.73 (s, 1 H); MS m / z (MH ⁺ ) 455.2.
[391] Method M: N- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -2-morpholin-4-yl- Acetam
[392] 2-Chloro-N- [3- (4-chloro-quinazolin-6-yl) -prop-2-ynyl] -acetamide:
[393] 2-Chloro-N-prop-2-ynyl-acetamide (385 mg, 2.93 mmol) and 4-chloro-6-iodoquinazoline (850 mg, 1 equiv) were dissolved in anhydrous THF and diisopropylamine (296 Mg, 0.41 mL, 1 equiv). To this mixture was added 0.04 equivalents of copper iodide (22 mg) and Pd (PPh ₃ ) ₂ Cl ₂ (82 mg). The reaction was stirred overnight (about 20 hours) at room temperature under nitrogen atmosphere. The solvent is then removed in vacuo and the residue is dissolved in CH ₂ Cl ₂ . The solution was transferred to a separatory funnel, washed once with saturated NH ₄ Cl and brine, dried over Na ₂ SO ₄ and the solvent removed in vacuo. The product was purified by silica gel chromatography eluting with 1: 1 hexanes / EtOAc and collecting fragments with Rf = 0.25. This gave 2-chloro-N- [3- (4-chloro-quinazolin-6-yl) -prop-2-ynyl] -acetamide as off-white solid. ¹ H NMR (400 MHz; CDCl ₃ ) δ 4.12 (2 H, s), 4.40 (2 H, d, J = 5.2 Hz), 7.91-7.93 (1 H, dd, J = 2, 6.8 Hz), 8.00 (1H, d, J = 8.4 Hz), 8.34 (1 H, d, J = 1.6 Hz), 9.03 (1 H, s). Irms (M &lt; + &gt;): 294.0, 296.0, 298.1.
[394] 2-Chloro-N- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl) -prop-2-ynyl] -acetamide:
[395] 2-chloro-N- [3- (4-chloro-quinazolin-6-yl) -prop-2-ynyl] -acet in 1,2-dichloroethane (1 mL) and t-butanol (1 mL) A solution of amide (50 mg, 0.17 mmol) and 3-methyl-4-phenoxyaniline (36 mg, 0.9 equiv) was heated at 87 ° C. for 30 minutes. The mixture was cooled to room temperature and diluted with ethyl acetate to facilitate further precipitation. The solution was then filtered to give the product coupled as a yellow powder (73 mg, 90%). 2.28 (3 H, s), 4.10 (2 H, s), 4.30 (2 H, s), 6.93 (3 H, d), 7.09 (1 H, t), 7.34 (2 H, t), 7.50- 7.53 (1 H, dd, J = 2.6, 6 Hz), 7.63 (1 H, d, J = 2.4 Hz), 7.78 (1 H, d, J = 8 Hz), 8.06-8.08 (1 H, dd, J = 1.4, 7.2 Hz), 8.68 (1 H, d, J = 1.2 Hz), 8.75 (1 H, s). Irms (M &lt; + &gt;): 457.0, 459.1; (M-): 455.7, 419.6.
[396] N- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl) -prop-2-ynyl] -2-morpholin-4-yl-acetamide:
[397] 2-Chloro-N- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl) -prop-2-ynyl] -acetamide in toluene (10 mL) To the solution (63 mg, 0.12 mmol) 3 equivalents of morpholine (31 mg) was added and the mixture was heated at reflux overnight. The reaction was cooled to room temperature, the morpholine salt was filtered off and the solvent was removed from the filtrate. The residue was redissolved in CH ₂ Cl ₂ with a small amount of methanol and HCl gas was forced out of the solution for 2-3 minutes. The solution is then concentrated to 2-3 ml, diluted with ethyl acetate and filtered to give N- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin- as a yellow / brown solid. 6-yl) -prop-2-ynyl] -2-morpholin-4-yl-acetamide was obtained (65 mg, 94%). ¹ H NMR (400 MHz; CD ₃ OD) δ 2.27 (3 H, s), 3.21 (2 H, m), 3.56 (2 H, m), 3.87 (2 H, m), 4.04 (2 H, m ), 4.09 (2 H, s), 4.36 (2 H, s), 6.93 (3 H, d, J = 8.4), 7.09 (1 H, t, J = 7.4 Hz), 7.34 (2 H, t, J = 8 Hz), 7.54 (1 H, dd), 7.65 (1 H, s), 7.82 (1 H, d, J = 8.8 Hz), 8.06 (1 H, d, J = 8.4 Hz), 8.76 ( 1 H, s), 8.80 (1 H, s). Irms (M &lt; + &gt;):508.0; (M-): 506.0.
[398] Method N: (3-Methyl-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-pyrido [3.4-d] pyrimidin-4-yl) amine
[399] 4,6-dichloro-pyrido [3,4-d] pyrimidine:
[400] DMF (0.1 mL) was added to 6-chloro-3H-pyrido [3,4-d] pyrimidin-4-one (1.82 g, 10 mmol) followed by thionyl chloride (10 mL). The flask was equipped with a condenser and drying tubes, and the contents were heated to reflux for about 20 minutes, whereby the solids dissolved. The heating was continued for another 1 hour, and then cooled. Toluene was added to wash the sides of the flask and the solvent was evaporated in vacuo. Repeated azeotrope twice with toluene and the crude product thus obtained was used in the next step.
[401] (6-Chloro-pyrido [3.4-d] pyrimidin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine:
[402] The 4,6-dichloro-pyrido [3,4-d] pyrimidine obtained from the reaction was dissolved in dioxane (50 mL) and 3-methyl-4-phenoxy aniline hydrochloride (2.8 g, 12 mmol) ) Was added and the contents were heated to an external bath temperature of about 80 ° C. for 3 hours, resulting in a yellow precipitate. Dioxane (20 mL) was further added and the contents heated at about 75 ° C. for 12 h. The solution is then filtered and the yellow solid is placed under vacuum to give the desired (6-chloro-pyrido [3.4-d] pyrimidin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine Hydrochloride was obtained (3.6 g, about 100%). ¹ H NMR (CD ₃ OD; 400 MHz) δ 9.05 (s, 1 H), 8.87 (s, 1 H), 8.64 (s, 1 H), 7.69 (d, J = 2.5 Hz, 1 H), 7.58 (dd, J = 8.7, 2.5 Hz, 1 H), 7.35 (dd, J = 8.7, 7.5 Hz, 2 H), 7.10 (t, J = 7.2 Hz, 1 H), 6.94 (d, J = 8.7 Hz , 3H), 2.29 (s, 3H). MS m / z (MH ⁺ ): 363.2.
[403] (3-Methyl-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-pyrido [3.4-d] pyrimidin-4-yl) -amine:
[404] (6-chloro-pyrido [3.4-d] pyrimidin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine hydrochloride in a flame dried pear shaped flask (200 mg, 0.5 mmol), 4-ethynyl-piperidine-1-carboxylic acid tert-butyl ester (314 mg, 1.5 mmol), Pd (PhCN) ₂ Cl ₂ (19 mg, 0.05 mmol), 1, Filled with 4-bis (diphenylphosphino) butane (32 mg, 0.075 mmol) and Cul (4.8 mg, 0.025 mmol). Dioxane (5 mL) was added and diisopropylamine (0.32 mL, 2.28 mmol) under Ar was added to this stirred suspension, thereby dissolving a large amount of solids. The flask (equipped with condenser) was then placed in a preheated oil-bath and heated for 14 hours at a bath temperature of 104 ° C. at which point LC / MS indicated loss of starting material. The reaction mixture was then filtered through a plug of silica, concentrated and chromatographed using a gradient elution of 20-80% EtOAc-hexanes to give the desired coupled product as a solid (165 mg, 62%). The solid is dissolved in CHCl (extra MeOH helps to dissolve), the HCl (g) is withdrawn and the solid is filtered off and precipitated as ether is added, which is then placed under vacuum to serve as the dihydrochloride salt. (3-methyl-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-pyrido [3.4-d] pyrimidin-4-yl) -amine was obtained. ¹ H NMR (CDCl ₃ ; 400 MHz) δ 9.12 (s, 1 H), 8.85 (s, 1 H), 8.68 (s, 1 H), 7.70 (d, J = 2.5 Hz, 1 H), 7.58 ( dd, J = 8.7, 2.5 Hz, 1 H), 7.34 (dd, J = 8.3, 7.5 Hz, 2 H), 7.10 (about t, J = 7.2 Hz, 1 H), 6.94 (d, J = 8.7 Hz , 3H), 3.42 (m, 2H), 3.19 (m, 3H), 2.29 (s, 3H), 2.22 (m, 2H), 2.0 (m, 2H). MS m / z (MH ⁺ ): 436.3.
[405] Method O: 4-Amino-4-methyl-1- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol
[406] 5- (4-Chloro-quinazolin-6-ylethynyl) -4,4-dimethyl-oxazolidin-2-one:
[407] 4,4-Dimethyl-5-ethynyl-2-oxazolidinone (1.10 g, 7.90 mmol), 4-chloro-6-iodoquinazolin (1.63 g, 5.60 mmol) in anhydrous THF (30 mL), dichloro A mixture of bis (triphenylphosphine) palladium (II) (200 mg, 0.28 mmol), copper iodide (53 mg, 0.28 mmol) and diisopropylamine (0.57 g, 5.60 mmol) at room temperature under nitrogen for 4 hours. Stirred. After concentration, the residue was dissolved in CH ₂ Cl ₂ (80 mL), washed with aqueous NH ₄ Cl and brine, dried over sodium sulfate and concentrated to afford the crude product as a brown oil. Purification by silica gel column using 50-70% EtOAc in hexanes yielded 1.22 g (72%) of a yellow solid. ¹ H NMR (CDCl ₃ ) δ 1.49 (s, 3 H), 1.53 (s, 3 H), 5.14 (s, 1 H), 5.57 (br s, 1 H), 7.95 (dd, 1 H), 8.04 (d, 1 H, J = 8.8 Hz), 8.38 (d, 1 H, J = 2.0 Hz), 9.05 (s, 1 H).
[408] 4-Amino-4-methyl-1- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol:
[409] 5- (4-Chloro-quinazolin-6-ylethynyl) -4,4-dimethyl-oxazolidin-2-one (151) in t-BuOH / ClCH ₂ CH ₂ Cl (1: 1, 2.0 mL) Mg, 0.5 mmol) and 3-methyl-4-phenoxyaniline hydrochloride (130 mg, 0.55 mmol) were heated in a capped reaction vial by intimate contact at 90 ° C. for 30 minutes. After cooling, the yellow mixture was diluted with EtOAc to precipitate more solids which were collected by suction filtration, rinsed with EtOAc and further dried to give 215 mg (86%) of a yellow solid. This material (215 mg, 0.43 mmol) was immediately mixed with KOH (0.51 g, 9.0 mmol) in MeOH / H ₂ O (9/3 mL) and refluxed for 20 hours. After cooling, the reaction was neutralized with 0.60 g (10.0 mmol) of AcOH and concentrated. The residue was suspended in CH ₂ Cl _2, then purified on silica gel column using a 20% MeOH in CH ₂ Cl _2. The purified free base was converted to HCl salt to give 46 mg (22%) of a yellow solid. ¹ H NMR (CD ₃ OD) δ 1.49 (s, 3 H), 1.52 (s, 3 H), 2.28 (s, 3 H), 4.64 (s, 1 H), 6.93 (m, 3 H), 7.09 (t, 1 H), 7.34 (m, 2 H), 7.55 (dd, 1 H), 7.65 (d, 1 H), 7.83 (d, 1 H), 8.13 (dd, 1 H), 8.77 (s , 1 H), 8.87 (s, 1 H); MS m / z (MH ⁺ ) 439.2.
[410] The following examples were made using the method described above. In the table below, the term "min" means minutes. Example numbers in the Tables below do not correspond to the compound numbers mentioned in the experimental section above.
[411] ExampleManufacturing methodIUPAC NameLRMS (MH +)HPLC retention time (minutes) OneI2-Methyl-4- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-2-ol396.16.88 2G '3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl]-(E) -prop-2-en-1-ol370.16.06 3B(6-cyclobutyl-quinazolin-4-yl)-(4-phenoxy-phenyl) -amine368.28.35 4B(6-cyclopropyl-quinazolin-4-yl)-(4-phenoxy-phenyl) -amine354.27.62 5I1-methoxy-2-methyl-4- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-2-ol426.16.66 6I4- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -2-pyridin-4-yl-but-3-yn-2-ol459.06.56 7I1- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -cyclohexanol436.17.80 8GN-Methyl-3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -acrylamide397.25.81 9G '3- [4- (4-benzyl-phenylamino) -quinazolin-6-yl]-(E) -prop-2-en-1-ol368.26.20 10G 'N, N-diethyl-3- [6- (3-hydroxy- (E) -propenyl) -quinazolin-4-ylamino] -benzamide377.24.28 11I4- [4- (4-benzyloxy-phenylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol465.14.88 12I4- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol538.2445.04.86 13I4- [4- (4-benzyl-phenylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol449.2356.25.11 14I1-Methyl-4- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol451.2143.24.89 15B3- (6-Cyclobutyl-quinazolin-4-ylamino) -N, N-diethyl-benzamide375.36.24
[412]
[413] 38I1- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -5-methoxy-3,5-dimethyl-hex-1-yn-3-ol555.27.55 39I4- [4- (4-benzyl-phenylamino) -quinazolin-6-yl] -2-pyridin-3-yl-but-3-yn-2-ol457.46.79 40I4- [4- (4-benzyl-phenylamino) -quinazolin-6-yl] -2-pyridin-4-yl-but-3-yn-2-ol457.36.71 41A(1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-dimethylamino-prop-1-ynyl) -quinazolin-4-yl) -amine482.25.16 42GAcetic acid 3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl]-(E) -allyl ester499.27.01 43C3- [4- (1-Benzellsulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -cyclobutanecarboxylic acid ethyl ester527.27.54 / 7.64 44I1-Methyl-4- [4- (1- (propane-2-sulfonyl) -1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol504.34.41 45H[6- [3- (6-Amino-3-aza-bicyclo [3.1.0] hex-3-yl (1a, 5a, 6a))-propenyl] -quinazolin-4-yl]-(1 -Benzenesulfonyl-1H-indol-5-yl) -amine537.24.42 46I2-Methyl-4- [4- (1- (propan-2-sulfonyl) -1H-indol-5-ylamino) -quinazolin-6-yl] -but-3-in-2-ol449.26.11 47I4- [4- (4-benzyloxy-phenylamino) -quinazolin-6-yl] -2-methyl-but-3-yn-2-ol410.36.63 48IN, N-diethyl-3- [6- (3-hydroxy-3-methyl-but-1-ynyl) -quinazolin-4-ylamino] -benzamide403.35.06 49I4- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -2-pyridin-4-yl-but-3-in-2-ol546.36.26 50D[3- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -cyclobutyl] -methanol485.25.97 51A(1-benzenesulfonyl-1H-indol-5-yl)-[6- [3- (2-methoxy-ethylamino) -prop-1-ynyl] -quinazolin-4-yl] -amine512.25.11 52A(1-benzenesulfonyl-1H-indol-5-yl)-[6- [3- (2-piperidin-1-yl-ethylamino) -prop-1-ynyl] -quinazolin-4- General] -amine563.25.23 53E[3- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -cyclobutyl] -pyrrolidin-1-yl-methanone552.26.45 / 6.64 54A(1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-morpholin-4-yl-prop-1-ynyl) -quinazolin-4-yl] -amine524.26.45 55A(1-benzenesulfonyl-1H-indol-5-yl)-[6- [3- (1,1-dioxo-1 & -thiomorpholin-4-yl) -prop-1-ynyl] -quina Zolin-4-yl] -amine572.26.36
[414] 56A(1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-methylamino-prop-1-ynyl) -quinazolin-4-yl] -amine468.24.89 57A(1-benzenesulfonyl-1H-indol-5-yl)-[6- [3- (2-morpholin-4-yl-ethylamino) -prop-1-ynyl) -quinazolin-4-yl ] -Amine567.25.05 58A(1-benzenesulfonyl-1H-indol-5-yl)-(6- [3- [3- (4-methyl-piperazin-1-yl) -propylamino] -prop-1-ynyl]- Quinazolin-4-yl) -amine594.24.41 59A(1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-pyrrolidin-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine508.35.21 60I4- [4- (1-Benz-1H-indazol-5-ylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol476.25.55 61I4- [4- (1-Benz-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol475.26.16 62I4- [4- (1-Cyclopropylmethyl-1H-indol-5-ylamino) -quinazolin-ylethynyl] -tetrahydro-pyran-4-ol439.35.82 63I4- [4- (1-ethanesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol477.25.34 64I4- [4- (1-Methanesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol463.24.99 65G '3- [4- (1-Benzyl-1H-indazol-5-ylamino) -quinazolin-6-yl]-(E) -acrylic acid methyl ester436.26.59 66I1- [4- (1-benzyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -cyclohexanol473.37.51 67I1- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -cyclohexanol523.37.37 68I4- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -1-methoxy-2-methyl-but-3-in-2-ol513.36.37 69I4- [4- (1-benzene-1H-indol-5-ylamino) -quinazolin-6-yl] -1-methoxy-2-methyl-but-3-in-2-ol463.36.43 70I4- [4- (1-Benz-1H-indol-5-ylamino) -quinazolin-6-yl] -2-pyridin-3-yl-but-3-yn-2-ol496.26.38 71J4- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol524.24.78 72J(1-benzenesulfonyl-1H-indol-5-yl)-[6-piperidin-4-ylethynyl-quinazolin-4-yl] -amine508.15.67 73J[6- (4-Amino-tetrahydro-pyran-4-ylethynyl) -quinazolin-4-yl]-(1-benzenesulfonyl-1H-indol-5-yl) -amine524.35.00
[415] 74I4- [4- (4-benzyloxy-phenylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol452.36.26 75I4- [4- [3-Methyl-4- (pyridin-2-ylmethoxy) -phenylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol467.35.24 76I1-Methyl-4- [4- [3-methyl-4- (pyridin-2-ylmethoxy) -phenylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol480.34.07 77J3- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol524.25.6 78I1-cyclopropyl-3- [4- [1- (propane-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-yl) -prop-2-yn-1-ol461.16.23 79I1-cyclopropyl-3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl) -prop-2-yn-1-ol408.27.00 80I4-Methyl-1- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol410.37.48 81I1- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol497.27.09 82I1- [4- (1-benzene-1H-indazol-5-ylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol448.36.58 83I4-Methyl-1- [4- (1-propane-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-yl] -pent-1-yn-3-ol463.26.69 84I1- [4- (4-benzyloxy-phenylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol424.27.31 85I4- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol437.24.81 86I4- [4- [4- (1-phenyl-ethoxy) -phenylamino] -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol466.26.46 87I1- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-yl] -4,4-dimethyl-pent-1-yn-3-ol462.37.00 88I4,4-Dimethyl-1- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol424.27.89
[416] 89I4,4-Dimethyl-1- [4- [1- (propane-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-yl] -pent-1-yn-3-ol477.27.12 90I1- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -4,4-dimethyl-pent-1-yn-3-ol511.27.51 91I1- [4- (4-benzyloxy-phenylamino) -quinazolin-6-yl] -4,4-dimethyl-pent-1-yn-3-ol438.27.74 92I4,4-Dimethyl-1- [4- [4- (1-phenyl-ethoxy) -phenylamino] -quinazolin-6-yl] -pent-1-yn-3-ol452.37.95 93J3- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol475.24.42 94JN, N-diethyl-3- [6- (3-hydroxy-piperidin-3-ylethynyl) -quinazolin-4-ylamino] -benzamide444.33.74 95J3- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol437.24.97 96J3- [4- (4-benzyloxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol451.34.94 97J3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol471.25.38 98J3- [4- (4-benzyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol435.25.16 99J3- [4- (1H-Indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol384.23.22 100I3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -1-cyclobutyl-prop-2-in-1-ol509.17.21 101I1-cyclobutyl-3- [4- (1-propane-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-yl] -prop-2-yn-1-ol475.26.81 102I3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-cyclobutyl-prop-2-yn-1-ol456.28.11 103I1-cyclobutyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-yn-1-ol436.27.95
[417] 104I3- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-yl] -1-cyclobutyl-prop-2-yn-1-ol460.26.69 105I1-cyclobutyl-3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-yn-1-ol422.27.59 106J3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-yn-1-ol451.25.26 107I3- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-3-ol538.24.92 108J3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol437.25.08 109J3- [4- (1-benzyl-1H-indol-5-ylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-in-1-ol474.25.00 110I5- [4- (4-benzyl-phenylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one449.27.03 111I4,4-Dimethyl-5- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -oxazolidin-2-one465.27.17 112I5- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one485.17.34 113I5- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one489.26.00 114I5- [4- (1-benzyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one488.26.58 115I5- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one538.16.21 116J3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol457.15.27 117J3- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol461.24.31
[418] 118J3- [4- (1- (Propan-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-ylethynyl] -pyrrolidin-3-ol476.14.35 119J3- [4- (3-benzyloxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol437.24.85 120J3- [4- (3-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol423.24.87 121J3- [4- (1-benzyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol460.04.81 123J3- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol510.24.82 124J3- [4- (1-benzyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol474.24.92 125J3- [4- (3-Methyl-4- (pyridin-2-ylmethoxy) -phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol466.24.14 126J3- [4- [1- (Propan-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol490.14.46 127J3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-yn-1-ol437.25.08 128J3- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-yn-1-ol475.24.45 129J3- [4- (1- (Propan-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2- In-1-ol490.24.52 130J3- [4- (4-benzyloxy-phenylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-yn-1-ol451.24.99 131J3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino] -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-in-1-ol524.24.94 132O4-Amino-1- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol459.15.41 133J3- [4- (3-Fluoro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol455.25.19
[419] 134J3- [4- (4-phenoxy-3-trifluoromethyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol505.15.61 135J4-Amino-1- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -pent-1-yn-3-ol498.14.82 136J3- [4- [4- (3-methoxy-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol481.25.15 137J3- [4- (3-Methyl-4-m-tolyloxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol465.15.56 138J3- [4- [4- (2-methoxy-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol481.14.94 139J3- [4- (3-Methyl-4-o-tolyloxy-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol465.25.50 140L3- [2- [4- (3-methyl-4-phenoxy-phenylamino] -quinazolin-6-yl] -ethyl] -piperidin-3-ol455.24.93 141J3- [4- [3-Chloro-4- (pyridin-2-ylmethoxy) -phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol486.04.38 142J3- [4- (5-Methyl-6-phenoxy-pyridin-3-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol452.04.70 143L3- [2- [4- (4-benzyl-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol439.24.81 144I5- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one481.26.64 145I1-Methyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol465.25.34 146L3- [2- [4- (1H-Indol-5-ylamino] -quinazolin-6-yl] -ethyl] -piperidin-3-ol388.32.86 147I3- [4- [3-methoxy-4-phenoxy-phenylamino] -quinazolin-6-ylethynyl] -1-methyl-piperidin-3-ol481.14.96
[420] 148I3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-3-ol485.15.48 149JEndo-α-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol497.15.47 150JEndo-α-3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol493.24.95 151JEndo-α-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol477.25.29 152JExo-β-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol497.15.35 153JExo-β-3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol493.24.86 154JExo-β-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol477.25.21 155JExo-β-3- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol463.24.96 156J(-)-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol451.25.22 157J(+)-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol451.25.22 158JEndo-α-3- [4- (4-phenoxy-phenylamino] -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol463.25.02 159J4- [4- (3-methoxy-4-phenoxy-phenylamino] -quinazolin-6-ylethynyl] -piperidin-4-ol467.24.77 160J4- [4- (3-Chloro-4-phenoxy-phenylamino] -quinazolin-6-ylethynyl] -piperidin-4-ol471.15.26 161J4- [4- (3-Methyl-4-phenoxy-phenylamino] -quinazolin-6-ylethynyl] -piperidin-4-ol451.25.09
[421] 162I4- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-7-ylethynyl] -tetrahydro-pyran-4-ol525.16.02 163I4- [4- (4-phenoxy-phenylamino) -quinazolin-7-ylethynyl] -tetrahydro-pyran-4-ol438.16.25 164J1- (3-Aza-bicyclo [3.1.0] hex-6-yl (1α, 5α, 6α))-3- [4- (3-methoxy-4-phenoxy-phenylamino] -quinazolin -6-yl] -prop-2-yn-1-ol479.15.73 165J1- (3-Aza-bicyclo [3.1.0] hex-6-yl (1α, 5α, 6α))-3- [4- (3-methyl-4-phenoxy-phenylamino] -quinazolin- 6-yl] -prop-2-yn-1-ol463.15.16 166J1- (3-Aza-bicyclo [3.1.0] hex-6-yl (1α, 5α, 6α))-3- [4- (4-phenoxy-phenylamino] -quinazolin-6-yl] -Prop-2-yn-1-ol449.04.89 167J3- [4- (4-phenoxy-phenylamino) -quinazolin-7-ylethynyl] -piperidin-3-ol437.25.09 168J3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-7-ylethynyl] -piperidin-3-ol467.24.97 169J3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-7-ylethynyl] -piperidin-3-ol471.15.48 170J3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-7-ylethynyl] -piperidin-3-ol451.25.35 171O4-Amino-1- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol455.24.91 172O4-Amino-4-methyl-1- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol439.25.26 173J3- [4- (3-ethynyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol369.24.11 174J3- [4- (3-Chloro-4-fluoro-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol397.14.43 175J[6- (4-Amino-tetrahydro-pyran-4-ylethynyl] -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine451.25.43
[422] 176J[6- (4-Amino-tetrahydro-pyran-4-ylethynyl] -quinazolin-4-yl]-(4-phenoxy-phenyl) -amine437.25.15 177J[6- (4-Amino-tetrahydro-pyran-4-ylethynyl] -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine467.25.00 178J(3-methoxy-4-phenoxy-phenyl)-(6-piperidin-2-ylethynyl-quinazolin-4-yl) -amine451.05.25 179J(3-Methyl-4-phenoxy-phenyl)-(6-piperidin-2-ylethynyl-quinazolin-4-yl) -amine435.05.71 180J(4-phenoxy-phenyl)-(6-piperidin-2-ylethynyl-quinazolin-4-yl) -amine421.25.32 181J(3-Chloro-4-phenoxy-phenyl)-(6-piperidin-2-ylethynyl-quinazolin-4-yl) -amine455.05.84 182J3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -piperidin-2-yl-prop-2-yn-1-ol451.25.16 183J3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -piperidin-2-yl-prop-2-yn-1-ol465.25.44 184J3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -piperidin-2-yl-prop-2-yn-1-ol485.15.58 185J3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -piperidin-2-yl-prop-2-yn-1-ol481.25.05 186J(4-phenoxy-phenyl)-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine421.25.27 187J(3-methoxy-4-phenoxy-phenyl)-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine451.25.21 188J(3-Chloro-4-phenoxy-phenyl)-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine455.05.79 189J3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -1-piperidin-3-yl-prop-2-yn-1-ol451.05.00
[423] 190J3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-piperidin-3-yl-prop-2-yn-1-ol465.05.26 191J3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-piperidin-3-yl-prop-2-yn-1-ol481.04.86 192J3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-piperidin-3-yl-prop-2-yn-1-ol485.05.34 193I1-Methyl-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl) -piperidin-4-ol465.05.18 194I4- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol485.05.34 195I4- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol481.04.81 196IN, N-diethyl-3- [6- (4-hydroxy-tetrahydro-pyran-4-ylethynyl) -quinazolin-4-ylamino] -benzeneamide445.34.66 197A(3- [3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -prop-2-ynyl] -3-aza-bicyclo [3.1 .0] hex-6-yl) -methanol550.35.38 198I4- [4- [1- (Propan-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol491.25.66 199I4- [4- (1H-Indol-5-ylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol385.24.22 200A1-Methyl-3- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol451.35.04 201J3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-yn-1-ol471.05.40 202I1- [4- (1-Benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -pent-1-yn-3-ol430.17.57 203I1- [4- [1- (Propan-2-sulfonyl) -1H-indol-5-ylamino) -quinazolin-6-yl] -pent-1-yn-3-ol449.26.28 204I1- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol430.17.57
[424] 205I1- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol410.27.39 206I1- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-yl] -pent-1-yn-3-ol434.26.16 207I1- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol396.27.04 208I3- [4- [4- (3-Fluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl) -piperidin-3-ol469.15.32 209J3- [4- [4- (4-methoxy-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl) -piperidin-3-ol481.25.10 210J4-Amino-1- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol425.25.13 211J4-Amino-1- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol410.34.86 212J4-Amino-1- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol445.25.27 213J4-amino-1- [4- (1- (propane-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-yl] -pent-1-yn-3-ol464.14.37 214J4-Amino-1- [4- [4- (3-fluoro-phenoxy) -3-methyl-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol443.25.25 215J3- [4- [4- (4-Fluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol469.25.28 216J3- [4- [4- (2-Fluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol469.25.22 217J3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol467.24.85 218J3- [4- [1- (3-Fluoro-benzyl) -1H-indazol-5-ylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol493.24.23 219J3- [4- [1- (3-methoxy-benzyl) -1H-indazol-5-ylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol505.114.41 220J3- [4- [1- (3-Methyl-benzyl) -1 H-indazol-5-ylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol489.24.70 221J3- [4- [1- (2-Fluoro-benzyl) -1H-indazol-5-ylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol493.24.47 222J2-Chloro-N, N-diethyl-4- [6- (3-hydroxy-piperidin-3-ylethynyl) -quinazolin-4-ylamino] -benzamide478.24.08 223J3- [4- (3-Bromo-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol516.05.41
[425] 224J3- [4- (3,5-Dichloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol506.15.64 225J3- [4- (3-Methyl-4-phenylsulfanyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol467.25.64 226J3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol453.24.76 227J4-Amino-1- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol441.24.78 228J1- [4- (3-methoxy-4-phenoxy-phenylamino] -quinazolin-6-yl] -pent-1-yn-3-ol426.26.83 229J3- [4- (4-benzenesulfinyl-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol483.14.08 230J3- [4- (4-benzenesulfonyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol485.14.49 231J3- [4- (4-benzenesulfinyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol469.13.89 232J3- [4- (4-benzenesulfonyl-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol499.14.65 233J3- [4- (4-cyclopentyloxy-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol443.25.30 234J3- [4- (4-cyclobutoxy-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol429.24.97 235J5- [6- (3-hydroxy-piperidin-3-ylethynyl) -quinazolin-4-ylamino] -2-phenoxy-benzonitrile462.24.86 236J3- [4- (4-cyclohexyloxy-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol457.35.62 237J3- [4- (4-phenylamino-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol436.24.58 238J3- [4- (3-phenyl-1H-indazol-6-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol461.23.86 239I4- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -2-methyl-but-3-yn-2-ol410.27.22 240I4- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -2-methyl-but-3-yn-2-ol430.17.39 241I4- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -2-methyl-but-3-yn-2-ol426.26.67 242I[6- (3-Methyl-but-1-ynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine394.29.08 243I(3-methoxy-4-phenoxy-phenyl)-[6- (3-methyl-but-1-ynyl) -quinazolin-4-yl] -amine410.28.47
[426] 244I(3-Chloro-4-phenoxy-phenyl)-[6- (3-methyl-but-1-ynyl) -quinazolin-4-yl] -amine414.19.21 245J3- [4- (4-benzyl-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol449.25.37 246J[6- (3-Amino-3-methyl-but-1-ynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine395.25.13 247J[6- (3-Amino-3-methyl-but-1-ynyl) -quinazolin-4-yl]-(4-phenoxy-phenyl) -amine409.25.45 248J[6- (3-Amino-3-methyl-but-1-ynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine425.25.04 249J[6- (3-Amino-3-methyl-but-1-ynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine429.15.56 250J[6- (3-Amino-prop-1-ynyl) -quinazolin-4-yl]-(4-phenoxy-phenyl) -amine367.24.78 251J[6- (3-Amino-prop-1-ynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine381.25.09 252J[6- (3-Amino-prop-1-ynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine397.24.72 253J[6- (3-Amino-prop-1-ynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine401.15.28 254J[6- (3-Methylamino-prop-1-ynyl) -quinazolin-4-yl]-(4-phenoxy-phenyl) -amine381.25.05 255J[6- (3-Methylamino-prop-1-ynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine395.25.32 256J(3-methoxy-4-phenoxy-phenyl)-[6- (3-methylamino-prop-1-ynyl) -quinazolin-4-yl] -amine411.24.87 257J(3-Chloro-4-phenoxy-phenyl)-[6- (3-methylamino-prop-1-ynyl) -quinazolin-4-yl] -amine415.15.45 258A[6- (3-Dimethylamino-prop-1-ynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine409.35.94 259J3- [4- (3-ethyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol465.25.54 260J3- [4- (3-Methyl-4-p-tolyloxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol465.25.52 261J3- [4- (3-hydroxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol453.14.34
[427] 262J2-Amino-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-1-ol411.24.95 263J2-Amino-4- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-1-ol427.14.60 264J3- [4- (3-ethoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol481.15.59 265J3- [4- (3-Isopropoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol495.25.40 266J3- [4- (2-Fluoro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol455.24.9 267J3- [4- (4-Fluoro-2-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol455.24.61 268J3- [4- (4-Pyridin-2-ylmethyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol436.23.59 269J2-Amino-1- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -cyclohexanol465.15.45 270J2-Amino-1- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -cyclohexanol481.25.10 271J1-Methylamino-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-2-ol425.25.08 272J4- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-methylamino-but-3-yn-2-ol441.24.76 273A '(3-Methyl-4-phenoxy-phenyl)-[6- (3-piperazin-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine450.05.25 274A(3-Methyl-4-phenoxy-phenyl)-[6- (3-pyrrolidin-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine435.05.95 275A '(3-methoxy-4-phenoxy-phenyl)-[6- (3-piperazin-1-yl-prop-1-ynyl) -quinazolin-4-yl] -amine466.34.95 276I3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -1-aza-bicyclo [2.2.2] octan-3-ol477.25.31 277J3- [4- [4- (2,6-Difluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol487.05.22 278A '[6- [3- (6-Amino-3-aza-bicyclo [3.1.0] hex-3-yl (1α, 5α, 6α))-prop-1-ynyl] -quinazolin-4-yl ]-(3-methyl-4-phenoxy-phenyl) -amine462.35.38 279A(3-Methyl-4-phenoxy-phenyl)-[6- (3-morpholin-4-yl-prop-1-ynyl) -quinazolin-4-yl] -amine451.07.27
[428]
[429]
[430]
[431]
[432] 351A4- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynylamino] -piperidine-1-carboxylic acid ethyl ester536.66.04 352A4- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynylamino] -piperidine-1-carboxylic acid ethyl ester552.35.97 353JN- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -isobutyramide451.37.09 354J[4- (2-Fluoro-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine453.45.55 355J[4- (3-Fluoro-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine453.45.75 356IN-Methyl-N- [3- [4- (4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide423.36.53 357IN-Methyl-N- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide437.36.86 358IN- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -N-methyl-acetamide457.37.05 359I2,2-Dimethyl-N- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -propionamide465.07.57 360J(3-Methyl-4-phenoxy-phenyl)-(6-pyrrolidin-3-ylethynyl-quinazolin-4-yl) -amine421.35.43 361J[4- (2-Fluoro-phenoxy) -3-methyl-phenyl]-(6-pyrrolidin-3-ylethynyl-quinazolin-4-yl) -amine439.05.39 362J(3-Chloro-4-phenoxy-phenyl)-(6-pyrrolidin-3-ylethynyl-quinazolin-4-yl) -amine441.05.61 363J(3-methoxy-4-phenoxy-phenyl)-(6-pyrrolidin-3-ylethynyl-quinazolin-4-yl) -amine437.15.06 364I2-Chloro-N- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide457.07.00 365ICyclopropanecarboxylic acid [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -amide449.16.97 366IN- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -propionamide437.16.74 367I2-methoxy-N- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide453.26.69
[433] 368MN- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -2-morpholin-4-yl-acetamide508.06.49 369A '1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperazin-2-carboxylic acid methyl ester508.05.66 370A4- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperazin-1-carboxylic acid amide493.55.87 371J(-)-(3-Methyl-4-phenoxy-phenyl]-(6-piperidin-3 (S) -ylethynyl-quinazolin-4-yl) -amine435.15.61 372A '4-Aminomethyl-1- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -pyrrolidin-3-ol480.34.95 373J4-hydroxy-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidine-2-carboxylic acid methylamide494.05.18 374N(3-Methyl-4-phenoxy-phenylamino)-(6-piperidin-3-ylethynyl-pyrido [3.4-d] -pyrimidin-4-yl) -amine436.35.40 375N(3-Methyl-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-pyrido [3.4-d] -pyrimidin-4-yl) -amine436.35.32 376J(3-methoxy-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl) -amine451.35.17 377J(3-Chloro-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl) -amine455.05.73 378J(3-Methyl-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl) -amine435.15.56 379A3 (S)-[4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidine-1-carboxylic acid methylamide492.37.15 380I3 (S)-[4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidine-1-carboxylic acid methylamide509.46.65 381IN- [1,1-dimethyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -2,2,2- Trifluoro-acetamide505.08.15 382J(+)-(3-Methyl-4-phenoxy-phenyl)-(6-piperidin-3 (R) -ylethynyl-quinazolin-4-yl) -amine435.35.61 383IN- [1,1-dimethyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide451.27.00
[434] 384IN- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1,1-dimethyl-prop-2-ynyl] -acetamide471.17.22 385J[4- (2-Chloro-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine469.05.97 386J[4- (2-Methoxy-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine465.15.31 387J[3-Methyl-4- (2-trifluoromethyl-phenoxy) -phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine503.06.17 388J[4- (2-Ethyl-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine463.06.38 389J(6-Azetidin-3-ylethynyl-quinazolin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine407.35.31 390IN- [1-Methyl-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide456.96.84 391IN- [1-Methyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide437.16.65 392IN- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide422.86.36
[435] Method I and appropriate starting materials (prepared according to methods known in the art) can be used to prepare the following compounds (and pharmaceutically acceptable salts and solvates thereof) that are part of the present invention:
[436] 1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -azetidin-1-yl] -ethanone,
[437] 1- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -azetidin-1-yl] -ethanone,
[438] 1- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -azetidin-1-yl] -ethanone,
[439] [6- (1-Methanesulfonyl-azetidin-3-ylethynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine,
[440] [6- (1-Methanesulfonyl-azetidin-3-ylethynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine,
[441] [6- (1-Methanesulfonyl-azetidin-3-ylethynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine,
[442] [6- (1-Methanesulfonyl-pyrrolidin-3-ylethynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine,
[443] [6- (1-Methanesulfonyl-pyrrolidin-3-ylethynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine,
[444] [6- (1-Methanesulfonyl-pyrrolidin-3-ylethynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine,
[445] 1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-1-yl] -ethanone,
[446] 1- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-1-yl] -ethanone,
[447] 1- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-1-yl] -ethanone,
[448] 1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-1-yl] -ethanone,
[449] 1- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-1-yl] -ethanone,
[450] 1- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-1-yl] -ethanone,
[451] [6- (1-Methanesulfonyl-piperidin-3-ylethynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine,
[452] [6- (1-Methanesulfonyl-piperidin-3-ylethynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine,
[453] [6- (1-Methanesulfonyl-piperidin-3-ylethynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine,
[454] 5- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-2-one,
[455] 5- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-2-one,
[456] 5- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-2-one,
[457] 4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-2-one,
[458] 4- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-2-one,
[459] 4- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-2-one,
[460] 1- [2- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -morpholin-4-yl] -ethanone,
[461] 1- [2- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -morpholin-4-yl] -ethanone,
[462] 1- [2- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -morpholin-4-yl] -ethanone,
[463] [6- (4-Methanesulfonyl-morpholin-2-ylethynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine,
[464] [6- (4-Methanesulfonyl-morpholin-2-ylethynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine,
[465] [6- (4-Methanesulfonyl-morpholin-2-ylethynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine,
[466] 6- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -morpholin-3-one,
[467] 6- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -morpholin-3-one,
[468] 6- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -morpholin-3-one,
[469] 5- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperazin-2-one,
[470] 5- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperazin-2-one,
[471] 5- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperazin-2-one,
[472] 6- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperazin-2-one,
[473] 6- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperazin-2-one,
[474] 6- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperazin-2-one,
[475] 1- [5- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,4-dihydro-2H-pyridin-1-yl] -ethanone ,
[476] 1- [5- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,4-dihydro-2H-pyridin-1-yl] -eta Paddy Field,
[477] 1- [5- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,4-dihydro-2H-pyridin-1-yl] -ethanone ,
[478] [6- (1-Methanesulfonyl-1,4,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) Amines,
[479] [6- (1-Methanesulfonyl-1,4,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl ) -Amine,
[480] [6- (1-Methanesulfonyl-1,4,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) Amines,
[481] 1- [5- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,6-dihydro-2H-pyridin-1-yl] -ethanone ,
[482] 1- [5- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,6-dihydro-2H-pyridin-1-yl] -eta Paddy Field,
[483] 1- [5- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,6-dihydro-2H-pyridin-1-yl] -ethanone ,
[484] [6- (1-Methanesulfonyl-1,2,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) Amines,
[485] [6- (1-Methanesulfonyl-1,2,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl ) -Amine,
[486] [6- (1-Methanesulfonyl-1,2,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) Amines,
[487] 1- [4- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,6-dihydro-2H-pyridin-1-yl] -ethanone ,
[488] 1- [4- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,6-dihydro-2H-pyridin-1-yl] -eta Paddy Field,
[489] 1- [4- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -3,6-dihydro-2H-pyridin-1-yl] -ethanone ,
[490] [6- (1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-ylethynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) Amines,
[491] [6- (1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-ylethynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl ) -Amine,
[492] [6- (1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-ylethynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) Amines,
[493] N- [1,1-dimethyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide,
[494] N- [1,1-dimethyl-3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide,
[495] N- [1,1-dimethyl-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide,
[496] N- [1,1-dimethyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -methanesulfonamide,
[497] N- [1,1-dimethyl-3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -methanesulfonamide,
[498] N- [1,1-dimethyl-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -methanesulfonamide,
[499] N- [1-Methyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide,
[500] N- [1-Methyl-3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide,
[501] N- [1-Methyl-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide,
[502] N- [1-Methyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -methanesulfonamide,
[503] N- [1-Methyl-3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -methanesulfonamide,
[504] N- [1-Methyl-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -methanesulfonamide,
[505] 1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperidin-2-one,
[506] 1- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperidin-2-one,
[507] 1- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperidin-2-one,
[508] 1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -pyrrolidin-2-one,
[509] 1- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -pyrrolidin-2-one, and
[510] 1- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -pyrrolidin-2-one.
[511] Method J and appropriate starting materials (prepared according to methods known in the art) can be used to prepare the following compounds (and pharmaceutically acceptable salts and solvates thereof) that are part of the present invention:
[512] (7-methoxy-6-piperidin-3-ylethynyl-quinazolin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine,
[513] (3-chloro-4-phenoxy-phenyl)-(7-methoxy-6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine,
[514] (3-methoxy-4-phenoxy-phenyl)-(7-methoxy-6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine,
[515] [7- (2-Methoxy-ethoxy) -6-piperidin-3-ylethynyl-quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine,
[516] (3-chloro-4-phenoxy-phenyl)-[7- (2-methoxy-ethoxy) -6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine,
[517] [7- (2-methoxy-ethoxy) -6-piperidin-3-ylethynyl-quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine,
[518] 3- [7- (2-methoxy-ethoxy) -4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol,
[519] 3- [7- (2-methoxy-ethoxy) -4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol,
[520] 3- [7- (2-methoxy-ethoxy) -4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol,
[521] 3- [7-methoxy-4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol,
[522] 3- [7-methoxy-4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol,
[523] 3- [7-methoxy-4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol,
[524] (6-azetidin-3-ylethynyl-quinazolin-4-yl)-(3-methoxy-4-phenoxy-phenyl) -amine,
[525] (3-methyl-4-phenoxy-phenyl)-(6-morpholin-2-ylethynyl-quinazolin-4-yl) -amine,
[526] (3-methoxy-4-phenoxy-phenyl)-(6-morpholin-2-ylethynyl-quinazolin-4-yl) -amine,
[527] (3-chloro-4-phenoxy-phenyl)-(6-morpholin-2-ylethynyl-quinazolin-4-yl) -amine,
[528] (3-methyl-4-phenoxy-phenyl)-[6- (1,4,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl] -amine,
[529] (3-methoxy-4-phenoxy-phenyl)-[6- (1,4,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl] -amine,
[530] (3-chloro-4-phenoxy-phenyl)-[6- (1,4,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl] -amine,
[531] (3-methyl-4-phenoxy-phenyl)-[6- (1,2,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl] -amine,
[532] (3-methoxy-4-phenoxy-phenyl)-[6- (1,2,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl] -amine,
[533] (3-Chloro-4-phenoxy-phenyl)-[6- (1,2,5,6-tetrahydro-pyridin-3-ylethynyl) -quinazolin-4-yl] -amine,
[534] (3-methyl-4-phenoxy-phenyl)-[6- (1,2,3,6-tetrahydro-pyridin-4-ylethynyl) -quinazolin-4-yl] -amine,
[535] (3-methoxy-4-phenoxy-phenyl)-[6- (1,2,3,6-tetrahydro-pyridin-4-ylethynyl) -quinazolin-4-yl] -amine,
[536] (3-chloro-4-phenoxy-phenyl)-[6- (1,2,3,6-tetrahydro-pyridin-4-ylethynyl) -quinazolin-4-yl] -amine,
[537] [6- (3-Amino-3-methyl-but-1-ynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine,
[538] [6- (3-Amino-3-methyl-but-1-ynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine,
[539] [6- (3-Amino-3-methyl-but-1-ynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine,
[540] [6- (3-Amino-but-1-ynyl) -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine,
[541] [6- (3-Amino-but-1-ynyl) -quinazolin-4-yl]-(3-methoxy-4-phenoxy-phenyl) -amine, and
[542] [6- (3-Amino-but-1-ynyl) -quinazolin-4-yl]-(3-chloro-4-phenoxy-phenyl) -amine.

权利要求:
Claims (35)
[1" claim-type="Currently amended] A compound of Formula 1, or a pharmaceutically acceptable salt or solvate thereof:
Formula 1

Where
X is N or CH;
A optionally contains 1 to 4 heteroatoms which may be the same or different and are selected from -N (R ¹ )-, O, and S (O) _j , where j is an integer from 0 to 2 A condensed 5, 6 or 7 membered ring containing a total of 1 to 3 double bonds, including those in the condensed pyridine or pyrimidine ring,
Wherein if the double bond comprises said selective nitrogen moiety -N (R ¹ )-then there is no R ¹ group bonded to nitrogen provided that the condensed ring does not form part of the purine and has two adjacent O or Does not contain an S (O) _j atom and the carbon atom of the A residue is optionally substituted with 1 to 3 R ⁵ groups;
R ¹ and R ² are each independently H or C ₁ -C ₆ alkyl;
R ³ is-(CR ¹ R ² ) _m -R ⁸ , wherein m is 0 or 1;
R ¹ and R ³ are together Forms a group of which is optionally substituted with one to three R ⁵ groups;
R ⁴ is-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _t R ⁹ ,-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _t R ⁹ ,- C = NOR ¹² or —X ¹ -R ¹² where m is an integer from 0 to 3, t is an integer from 0 to 5, and X ¹ is an azetidine, oxetane or C ₃ -C ₄ carbocyclic group Is a group derived from) or-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _k R ¹³ or-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _k R ¹³ (where k is an integer from 1 to 3, m is an integer from 0 to 3), or-(CR ¹ R ² ) _t R ⁹ (where t is an integer from 0 to 5, the attachment point to R ⁹ is through a carbon atom of the R ⁹ groups shown below);
Each R ⁵ is independently halo, hydroxy, —NR ¹ R ² , C ₁ -C ₆ alkyl, trifluoromethyl, C ₁ -C ₆ alkoxy, trifluoromethoxy, -C (O) R ⁶ ,- CO ₂ R ⁶ , -NR ⁶ C (O) R ¹ , -C (O) NR ⁶ R ⁷ , -SO ₂ NR ⁶ R ⁷ , -NR ⁶ C (O) NR ⁷ R ¹ and -NR ⁶ C ( O) OR ⁷ ;
R ⁶ and R ⁷ are each independently H, C ₁ -C ₆ alkyl,-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _t (4- to 10-membered heterocycle) Wherein t is an integer from 0 to 5 and one or two ring carbon atoms of the heterocyclic group are optionally substituted with an oxo (═O) moiety and alkyl of the above R ⁶ and R ⁷ groups , Aryl and heterocyclic moieties include halo, cyano, nitro, -NR ¹ R ² , trifluoromethyl, trifluoromethoxy, C ₁ -C ₆ alkyl, C ₂ -C ₆ alkenyl, C ₂ -C ₆ Optionally substituted with 1 to 3 substituents independently selected from alkynyl, hydroxy and C ₁ -C ₆ alkoxy;
R ⁸ is independently-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _t (4 to 10 membered heterocycle), wherein t is an integer from 0 to 5, One or two ring carbon atoms of the heterocyclic group are optionally substituted with oxo (═O) residues), wherein the R ⁸ groups are each optionally substituted with 1 to 5 R ¹⁰ groups;
R ⁹ is a non-aromatic monocyclic ring, a condensed or crosslinked bicyclic ring, or a spiro ring ring, wherein the ring has 0 to 3 carbon atoms N, O, S (O) _j , wherein j is 0 to 2 Integer) and 3 to 12 carbon atoms optionally substituted with a hetero residue independently selected from -NR ^12- , provided that only two O atoms, two S (O) _j residues, an O atom and S (O ) _j residues, N and S atoms, or N and O atoms are not directly bonded to each other in the ring, and the carbon atoms of the ring are optionally substituted with 1 or 2 R ¹¹ groups;
Each R ¹⁰ is independently halo, cyano, nitro, trifluoromethoxy, trifluoromethyl, azido, hydroxy, C ₁ -C ₆ alkoxy, C ₁ -C ₁₀ alkyl, C ₂ -C ₆ alkenyl , C ₂ -C ₆ alkynyl, -C (O) R ⁶ , -C (O) OR ⁶ , -OC (O) R ⁶ , -NR ⁶ C (O) R ⁷ , -NR ⁶ C (O) NR ¹ R ⁷ , -NR ⁶ C (O) OR ⁷ , -C (O) NR ⁶ R ⁷ , -NR ⁶ R ⁷ , -NR ⁶ OR ⁷ , -SO ₂ NR ⁶ R ⁷ , -S (O) _j (C ₁ -C ₆ alkyl), where j is an integer from 0 to 2,-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl),-(CR ¹ R ² ) _t (4 to 10 membered heterocycle),-(CR ¹ R ² ) _q C (O) (CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl),-(CR ¹ R ² ) _q C (O) (CR ¹ R ² ) _t (4 to 10 membered heterocycle),-(CR ¹ R ² ) _t O (CR ¹ R ² ) _q (C ₆ -C ₁₀ aryl),-(CR ¹ R ² ) _t O (CR ¹ R ² ) _q (4 to 10 membered heterocycle),-(CR ¹ R ² ) _q S (O) _j (CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _q S (O) _j (CR ¹ R ² ) _t (4 to 10 membered heterocycle), wherein j is 0 to 2 and q and t are each independently an integer of 0 to 5, and
One or two ring carbon atoms of the heterocyclic residue of the R ¹⁰ group is optionally substituted with an oxo (═O) residue,
Alkyl, alkenyl, alkynyl, aryl and heterocyclic moieties of the above R ¹⁰ group include halo, cyano, nitro, trifluoromethyl, trifluoromethoxy, azido, -OR ⁶ , -C (O) R ⁶ , -C (O) OR ⁶ , -OC (O) R ⁶ , -NR ⁶ C (O) R ⁷ , -C (O) NR ⁶ R ⁷ , -NR ⁶ R ⁷ , -NR ⁶ OR ⁷ , C ₁ -C ₆ alkyl, C ₂ -C ₆ alkenyl, C ₂ -C ₆ alkynyl,-(CR ¹ R ² ) _t (C ₆ -C ₁₀ aryl) and-(CR ¹ R ² ) _t (4 Optionally substituted with 1 to 3 substituents independently selected from: 10-membered heterocycle), wherein t is an integer from 0 to 5;
R ¹¹ are each independently -R ¹² , -OR ¹ , -NR ¹ R ² , -NR ⁶ C (O) R ⁷ , -NR ⁶ C (O) NR ⁷ R ¹ , -NR ⁶ C (O) OR ⁷ and —NR ⁶ SO ₂ NR ⁷ R ¹ , or substitute two hydrogen atoms on carbon to form an oxo (C═O) group;
R ¹² is R ⁶ , —C (O) R ⁶ or —SO ₂ R ⁶ , —C (O) NR ⁶ R ⁷ , —SO ₂ NR ⁶ R ⁷ or —CO ₂ R ⁶ ;
R ¹³ is —NR ¹ R ¹² or —OR ¹² ;
Wherein any substituent described above including a halogeno, SO or SO ₂ group, or a CH ₃ (methyl), CH ₂ (methylene) or CH (methine) group not bonded to an N, O or S atom Optionally has a substituent selected from hydroxy, halo, C ₁ -C ₄ alkyl, C ₁ -C ₄ alkoxy and —NR ¹ R ² in the group.
[2" claim-type="Currently amended] The method of claim 1,
A moiety selected from the following formula, wherein R has a R ⁴ group as a substituent, and optionally 1 to 3 R ⁵ groups as a substituent:

[3" claim-type="Currently amended] The method of claim 1,
A moiety selected from the following formula, wherein R has a R ⁴ group as a substituent, and optionally 1 to 3 R ⁵ groups as a substituent:

[4" claim-type="Currently amended] The method of claim 1,
A moiety selected from the following formula, wherein R has a R ⁴ group as a substituent, and optionally 1 to 3 R ⁵ groups as a substituent:

[5" claim-type="Currently amended] The method of claim 1,
A moiety selected from the following formula, wherein R has a R ⁴ group as a substituent, and optionally 1 to 3 R ⁵ groups as a substituent:

[6" claim-type="Currently amended] The method of claim 1,
A moiety having the formula: wherein R has a R ⁴ group as a substituent and optionally 1 to 3 R ⁵ groups as a substituent:

[7" claim-type="Currently amended] The method of claim 5,
R ⁴ is-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _t R ⁹ , wherein m is an integer of 0 to 3 and t is an integer of 0 to 5;
[8" claim-type="Currently amended] The method of claim 6,
R ⁴ is-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _t R ⁹ , wherein m is an integer of 0 to 3 and t is an integer of 0 to 5;
[9" claim-type="Currently amended] The method of claim 5,
R ⁴ is-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _t R ⁹ , wherein m is an integer of 0 to 3 and t is an integer of 0 to 5;
[10" claim-type="Currently amended] The method of claim 6,
R ⁴ is-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _t R ⁹ , wherein m is an integer of 0 to 3 and t is an integer of 0 to 5;
[11" claim-type="Currently amended] The method of claim 5,
R ⁴ is-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _k R ¹³ or-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _k R ¹³ where , m is an integer of 0 to 3, k is an integer of 1 to 3).
[12" claim-type="Currently amended] The method of claim 6,
R ⁴ is-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _k R ¹³ or-(CR ¹ R ² ) _m -C = C- (CR ¹ R ² ) _k R ¹³ where , m is an integer of 0 to 3, k is an integer of 1 to 3).
[13" claim-type="Currently amended] The method of claim 5,
R ⁴ is -C = NOR ¹² or -X ¹ -R ¹² , wherein X ¹ is a divalent group derived from an azetidine, oxetane or C ₃ -C ₄ carbocyclic group, or-(CR ¹ R ²⁾ _t R ⁹ (where the attachment point to R ⁹ is through a carbon atom of the R ⁹⁾ compounds.
[14" claim-type="Currently amended] The method of claim 6,
R ⁴ is -C = NOR ¹² or -X ¹ -R ¹² , wherein X ¹ is a divalent group derived from an azetidine, oxetane or C ₃ -C ₄ carbocyclic group, or-(CR ¹ R ²⁾ _t R ⁹ (where the attachment point to R ⁹ is through a carbon atom of the R ⁹⁾ compounds.
[15" claim-type="Currently amended] The method of claim 7, wherein
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[16" claim-type="Currently amended] The method of claim 8,
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[17" claim-type="Currently amended] The method of claim 9,
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[18" claim-type="Currently amended] The method of claim 10,
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[19" claim-type="Currently amended] The method of claim 11,
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[20" claim-type="Currently amended] The method of claim 12,
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[21" claim-type="Currently amended] The method of claim 13,
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[22" claim-type="Currently amended] The method of claim 14,
R ⁸ is-(CR ¹ R ² ) _t (phenyl),-(CR ¹ R ² ) _t (pyridyl),-(CR ¹ R ² ) _t (pyrimidinyl),-(CR ¹ R ² ) _t (Indolyl),-(CR ¹ R ² ) _t (indazolyl) and-(CR ¹ R ² ) _t (benzimidazolyl), where t is an integer from 0 to 5, wherein the R ⁸ group is Each optionally substituted with 1 to 5 R ¹⁰ groups.
[23" claim-type="Currently amended] The method of claim 16,
The variable m in group R ⁴ is 0,
T is an integer from 0 to 2 in the group R ⁸ ,
R ⁹ is a 4 to 10 membered heterocyclic group having 1 to 3 hetero moieties as described in claim 1 and optionally substituted with 1 or 2 R ¹¹ groups.
[24" claim-type="Currently amended] The method of claim 20,
R ⁴ is-(CR ¹ R ² ) _m -C≡C- (CR ¹ R ² ) _k R ¹³ , wherein m is 0 and k is an integer from 0 to 2.
[25" claim-type="Currently amended] The method of claim 22,
R ⁴ is _{^{- (CR 1 R 2) t}} R 9 , provided wherein the attachment point to R ⁹ is a cylindrical carbon atom of the R ^9, and, t is an integer from 0 to 2, R ⁹ is as described in claim 1, wherein A 4-10 membered heterocyclic group having 1 to 3 hetero moieties and optionally substituted with 1 or 2 R ¹¹ groups.
[26" claim-type="Currently amended] The method of claim 1,
A compound selected from the group consisting of:
Acetic acid 3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -allyl ester;
(1-benzenesulfonyl-1H-indol-5-yl)-[6- [3- (4-methyl-piperazin-1-yl) -prop-1-ynyl] -quinazolin-4-yl] Amines;
(1-benzenesulfonyl-1H-indol-5-yl)-[6- (3-pyrrolidin-1-yl-prop-1-ynyl] -quinazolin-4-yl] -amine;
4- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol;
(1-benzenesulfonyl-1H-indol-5-yl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl] -amine;
[6- (4-Amino-tetrahydro-pyran-4-ylethynyl) -quinazolin-4-yl]-(1-benzenesulfonyl-1H-indol-5-yl) -amine;
1-methyl-4- [4- [3-methyl-4- (pyridin-2-ylmethoxy) -phenylamino] -quinazolin-6-ylethynyl] -piperidin-4-ol;
1- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol;
4- [4- [4- (1-phenyl-ethoxy) -phenylamino] -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol;
1- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-yl] -4,4-dimethyl-pent-1-yn-3-ol;
4,4-dimethyl-1- [4- [4- (1-phenyl-ethoxy) -phenylamino] -quinazolin-6-yl] -pent-1-yn-3-ol;
3- [4- [1- (Propan-2-sulfonyl) -1H-indol-5-ylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol;
1-methyl-3- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-pyrrolidin-2-yl-prop-2-yn-1-ol;
5- [4- (1-benzyl-1H-indazol-5-ylamino) -quinazolin-6-ylethynyl] -4,4-dimethyl-oxazolidin-2-one;
4-amino-1- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -pent-1-yn-3-ol;
4-amino-1- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -4-methyl-pent-1-yn-3-ol;
3- [2- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol; And
Pharmaceutically acceptable salts and solvates of these compounds.
[27" claim-type="Currently amended] The method of claim 1,
A compound selected from the group consisting of:
(+)-(3-Methyl-4-phenoxy-phenyl)-(6-piperidin-3 (R) -ylethynyl-quinazolin-4-yl) -amine;
(-)-(3-methyl-4-phenoxy-phenyl)-(6-piperidin-3 (S) -ylethynyl-quinazolin-4-yl) -amine;
3- (S)-[4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidine-1-carboxylic acid methylamide;
3- (S)-[4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidine-1-carboxylic acid methylamide;
(3-Methyl-4-phenoxy-phenyl)-(6-pyrrolidin-3-ylethynyl-quinazolin-4-yl) -amine;
3- [4- (5-Methyl-6-phenoxy-pyridin-3-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
(-)-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
(+)-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
4- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -tetrahydro-pyran-4-ol;
[6- [1- (2-methoxy-ethyl) -piperidin-3-ylethynyl] -quinazolin-4-yl]-(3-methyl-4-phenoxy-phenyl) -amine;
[4- (2-Fluoro-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine;
[4- (3-Fluoro-phenoxy) -3-methyl-phenyl]-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine;
(6-azetidin-3-ylethynyl-quinazolin-4-yl)-(3-methyl-4-phenoxy-phenyl) -amine;
3- [4- [4- (2-fluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol;
3- [4- [4- (3-fluoro-phenoxy) -3-methyl-phenylamino] -quinazolin-6-ylethynyl] -piperidin-3-ol;
[4- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-4-ol;
(3-Chloro-4-phenoxy-phenyl)-(6-piperidin-3-ylethynyl-quinazolin-4-yl) -amine;
3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -8-aza-bicyclo [3.2.1] octan-3-ol;
(3-Chloro-4-phenoxy-phenyl)-(6-piperidin-4-ylethynyl-quinazolin-4-yl) -amine;
3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -pyrrolidin-3-ol;
3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-7-ylethynyl] -piperidin-3-ol; And
Pharmaceutically acceptable salts and solvates of these compounds.
[28" claim-type="Currently amended] The method of claim 1,
A compound selected from the group consisting of:
N- [3- [4- (3-Chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
N- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
(3- [3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -3-aza-bicyclo [3.1.0] Hex-6-yl) -methanol;
4- [3- [4- (3-methoxy-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperazin-1-carboxylic acid methylamide;
[6- [3- (1,1-Dioxo-1-thiomorpholin-4-yl) -prop-1-ynyl] -quinazolin-4-yl]-(3-methyl-4-phenoxy -Phenyl) -amine;
1- [3- [4- (3-Methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -piperidin-4-ol;
N- [1-Methyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
N-3- [4- (3-chloro-4-phenoxy-phenylamino) -quinazolin-6-yl] -1-methyl-prop-2-ynyl] -acetamide;
N- [1,1-Dimethyl-3- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -prop-2-ynyl] -acetamide;
4- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -1-methyl-piperidin-4-ol;
3- [4- (1-benzenesulfonyl-1H-indol-5-ylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
3- [4- (3-bromo-4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
3- [4- (4-benzenesulfonyl-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
3- [4- (4-cyclohexyloxy-3-methyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
2-methyl-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-2-ol;
2-amino-4- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -but-3-yn-1-ol;
3- [4- (3-Methyl-4-phenylsulfanyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol; And
Pharmaceutically acceptable salts and solvates of these compounds.
[29" claim-type="Currently amended] The method of claim 1,
A compound selected from the group consisting of:
3- [4- (3-chloro-4-fluoro-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
3- [4- (3-ethynyl-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
(3-methyl-4-phenoxy-phenyl)-[6- (1-methyl-piperidin-3-ylethynyl) -quinazolin-4-yl] -amine;
(3-methyl-4-phenoxy-phenyl)-[6- (2-piperidin-3-yl-ethyl) -quinazolin-4-yl] -amine;
3- [2- [4- (3-methyl-4-phenoxy-phenylamino) -quinazolin-6-yl] -ethyl] -piperidin-3-ol;
3- [4- (4-phenoxy-phenylamino) -quinazolin-6-ylethynyl] -piperidin-3-ol;
3-oxo-5- (4-pyrrolidin-1-yl-butyl) -1,2,3,5-tetrahydro-benzo [4,5] imidazo [1,2-a] pyridine-4- Carboxylic acid benzylamide; And
Pharmaceutically acceptable salts and solvates of these compounds.
[30" claim-type="Currently amended] A method for treating abnormal cell growth in a mammal comprising administering to the mammal an amount of a compound according to claim 1 effective to treat abnormal cell growth.
[31" claim-type="Currently amended] The method of claim 30,
How abnormal cell growth is cancer.
[32" claim-type="Currently amended] The method of claim 31, wherein
The cancer may be lung cancer, bone cancer, pancreatic cancer, skin cancer, head or neck cancer, skin or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer, cancer of the anal region, gastric cancer, colon cancer, breast cancer, fallopian tube carcinoma, uterus Endometrial carcinoma, cervical carcinoma, vaginal carcinoma, vulvar carcinoma, Hodgkin's disease, esophageal cancer, small intestine cancer, endocrine cancer, thyroid cancer, parathyroid cancer, adrenal gland cancer, soft tissue sarcoma, urethral cancer, penis cancer, prostate cancer, chronic cancer Or acute leukemia, lymphocytic lymphoma, bladder cancer, renal or ureter cancer, renal cell carcinoma, renal carcinoma, central nervous system (CNS) neoplasm, primary CNS lymphoma, spinal cord tumor, brain stem glioma, pituitary adenoma, or these Selected from one or more combinations of cancers.
[33" claim-type="Currently amended] The method of claim 31, wherein
An effective amount of the compound of claim 1 to treat abnormal cell growth is a mitotic inhibitor, an alkylating agent, an anti-metabolic agent, an insert antibiotic, a growth factor inhibitor, an emitter, a cell circulation inhibitor, an enzyme, a topoisomerase inhibitor, a biological agent. A method for treating abnormal cell growth in a mammal comprising administering to the mammal with an anti-tumor agent selected from the group consisting of response modifiers, antibodies, cytotoxic agents, anti-hormonal agents and anti-androgens.
[34" claim-type="Currently amended] A pharmaceutical composition for treating abnormal cell growth in a mammal comprising an amount of the compound according to claim 1 and a pharmaceutically acceptable carrier effective to treat abnormal cell growth.
[35" claim-type="Currently amended] (a) reacting a compound of formula 11 or formula 2 with a compound of formula 3, or (b) reacting a compound of formula 7 with a compound of formula 3 to obtain an intermediate of formula 5 A process for preparing the compound of formula 1 according to claim 1

Where
Z is a leaving group and A, X, R ¹ , R ⁴ and R ³ are as defined in claim 1.

Where
X, R ¹ , A and R ³ are as defined in claim ¹ and Z ¹ is an activation group.

Where
Z ¹ is activated group, and, X, R ^1, A and R ³ are as defined in claim 1, Z ¹ is converted to R ⁴ groups are to be converted to the R ⁴ group is optionally further R ⁴ group, Can be.

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引用文献:

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公开号 | 申请日 | 公开日 | 申请人 | 专利标题

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法律状态:
1999-01-27|Priority to US11734699P

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1999-01-27|Priority to US60/117,346

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1999-12-06|Application filed by 실버스타인 아써 에이., 화이자 프로덕츠 인코포레이티드

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2001-11-14|Publication of KR20010101774A

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2005-02-21|Application granted

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2005-02-21|Publication of KR100471953B1

优先权:

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申请号 | 申请日 | 专利标题

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US11734699P| true| 1999-01-27|1999-01-27|

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US60/117,346|1999-01-27|